Recombinant human adenovirus type 5 based vaccine candidates against GIIa- and GIIb-genotype porcine epidemic diarrhea virus induce robust humoral and cellular response in mice

文献类型： 外文期刊

第一作者： Miao, Xin

作者： Miao, Xin;Zhang, Liping;Zhou, Peng;Zhang, Zhongwang;Yu, Ruiming;Liu, Xiaoqing;Lv, Jianliang;Wang, Yonglu;Guo, Huichen;Pan, Li;Liu, Xinsheng

作者机构：

关键词： Immunogenicity; Porcine epidemic diarrhea virus (PEDV); Ad5-vector vaccine; Spike protein; Oral

期刊名称：VIROLOGY （ 影响因子：3.7； 五年影响因子：3.2 ）

ISSN： 0042-6822

年卷期： 2023 年 584 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) is a porcine enteropathogenic coronavirus causing severe watery diarrhea, vomiting, dehydration, and death in piglets. However, most commercial vaccines are developed based on the GI genotype strains, and have poor immune protection against the currently dominant GII genotype strains. Therefore, four novel replication-deficient human adenovirus 5-vectored vaccines expressing codonoptimized forms of the GIIa and GIIb strain spike and S1 glycoproteins were constructed, and their immunogenicity was evaluated in mice by intramuscular (IM) injection. All the recombinant adenoviruses generated robust immune responses, and the immunogenicity of recombinant adenoviruses against the GIIa strain was stronger than that of recombinant adenoviruses against the GIIb strain. Moreover, Ad-XT-tPA-Sopt-vaccinated mice elicited optimal immune effects. In contrast, mice immunized with Ad-XT-tPA-Sopt by oral gavage did not induce strong immune responses. Overall, IM administration of Ad-XT-tPA-Sopt is a promising strategy against PEDV, and this study provides useful information for developing viral vector-based vaccines.

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