Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton

文献类型： 外文期刊

第一作者： Fei, Xiaowen

作者： Fei, Xiaowen;Xiao, Sha;Huang, Xiaodan;Li, Zhijie;He, Changhao;Li, Xinghan;Li, Yajun;Zhang, Xiuxia;Deng, Xiaodong;Li, Xinghan;Li, Yajun;Zhang, Xiuxia;Deng, Xiaodong;Li, Yajun;Zhang, Xiuxia;Deng, Xiaodong;Deng, Xiaodong

作者机构：

期刊名称：PLOS NEGLECTED TROPICAL DISEASES （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN： 1935-2735

年卷期： 2023 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Author summaryA new low-cost strategy was developed to control vector mosquitoes safely. Chlorella was genetically modified to produce siRNA targeting the mosquito chsa gene, inhibiting gene expression after the larval stage. The inhibition prevented metamorphosis with high larval mortality in both Aedes aegypti and Aedes albopictus. The recombinant Chlorella performed well in the simulated field trials but impacted plankton populations in the released water, which indicates that the technology requires further optimization for field use. This approach is promising for biorational mosquito control to prevent mosquito-borne diseases. New biocontrol strategies are urgently needed to combat vector-borne infectious diseases. This study presents a low-cost method to produce a potential mosquito insecticide that utilizes the microalgae released into suburban water sources to control mosquito populations. Chlorella microalgae are ubiquitous in local waters, which were chosen as the host for genetic transfection. This species facilitated the recombinant algae to adapt to the prevailing environmental conditions with rapid growth and high relative abundance. The procedure involved microalgae RNAi-based insecticides developed using short hairpin RNAs targeting the Aedes aegypti chitin synthase A (chsa) gene in Chlorella. These insecticides effectively silenced the chsa gene, inhibiting Aedes metamorphosis in the laboratory and simulated-field trials. This study explored the impact of recombinant microalgae on the phytoplankton and zooplankton in suburban waters. High-throughput sequencing revealed that rapid reproduction of recombinant Chlorella indirectly caused the disappearance of some phytoplankton and reduced the protozoan species. This study demonstrated that a recombinant microalgae-based insecticide could effectively reduce the population of Aedes mosquitoes in the laboratory and simulated field trials. However, the impact of this technology on the environment and ecology requires further investigation.

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