Activated charcoal supplementation in cottonseed meal-based feed improved growth performance and antioxidant capacity through enhancing intestinal barrier function in grass carp juveniles ( Ctenopharyngodon idellus)

文献类型： 外文期刊

第一作者： Liu, Hengchen

作者： Liu, Hengchen;Gu, Zhengyan;Lin, Yan;Jiang, Wenqiang;Lu, Siyue;Miao, Linghong;Liu, Hengchen;Kassaw, Mohammed Kebede;Chen, Shiyou;Qian, Linjie;Miao, Linghong;Shao, Shanshan;Li, Siming

作者机构：

关键词： Herbivorous fish; Cottonseed meal-based feed; Activated charcoal; Growth performance; Intestinal barrier and liver antioxidant capacity

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN： 2352-5134

年卷期： 2024 年 39 卷

页码：

收录情况： SCI

摘要： To evaluate the efficiency of activated charcoal (AC) in cottonseed meal-based feed (CSM; 38.46 % CSM, 1275 mg/kg free gossypol), experimental diets supplemented with 0 % AC, 1.5 % AC and 3.0 % AC were prepared and used to feed grass carp juveniles ( Ctenopharyngodon idellus, initial weight of 5.0 +/- 0.5 g) for 8 weeks. The results showed that the 1.5 % AC group significantly increased final body weight (FBW), weight growth rate (WGR), specific growth rate (SGR), and activated the expressions of intestinal barrier-associated genes zonula occludens-1 (zo-1), zo-2 and claudin-c compared with 0 % AC group (P < 0.05). Additionally, the 1.5 % AC and the 3.0 % AC groups significantly promoted the expression of claudin-12 (P < 0.05). Compared with the 0 % AC group, the 1.5 % AC group had significantly higher total protein (TP) concentration and significantly lower glucose (GLU) concentration and aspartate aminotransferase (AST) activity (P < 0.05). Liver antioxidant results showed that supplementation with 1.5 % AC and 3.0 % AC groups significantly reduced malondialdehyde (MDA) content and increased glutathione reductase (GR) activity (P < 0.05). Total-superoxide dismutase (T-SOD) and glutathione-S-transferase (GST) activities were significantly higher in the 1.5 % AC group than those in the other groups (P < 0.05). The antioxidant gene results showed that gr and GST omega 1 ( gsto1 ) levels were significantly higher in the 3.0 % AC group than in the 0 % AC group (P < 0.05). The 1.5 % AC group significantly promoted nuclear factor erythroid 2-related factor 2 ( nrf2 ) expression, and the 1.5 % AC and 3.0 % AC groups significantly inhibited the expressions of kelch-like ECH-associated protein 1 ( keap1a ) and keap1b (P < 0.05). Liver apoptotic and inflammatory genes showed that the expressions of caspase 9 ( casp9 ), BCL2 associated X a (baxa), nuclear factor-kappa B ( nf kappa b ) and inhibitor of kappa B (i kappa b) were significantly lower in the 1.5 % AC group than those in the other groups (P < 0.05). The expression of casp8 in the 1.5 % AC group was significantly lower than in the 0 % AC group (P < 0.05). Additionally, the expressions of p38 mitogen-activated protein kinase ( p38MAPK ) and i kappa b kinase-beta ( ikk beta ) were significantly suppressed in the 1.5 % AC group and 3.0 % AC group (P < 0.05). In summary, 1.5 % AC supplementation in CSM-based feed can enhance intestinal barrier function, alleviate CSMbased feed-induced apoptosis, improve liver antioxidant, and thus promote grass carp weight growth rate.

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