Biological characterization and genomic profiling of a novel Providencia phage isolated from farm effluents

文献类型： 外文期刊

第一作者： Zhu, Mantong

作者： Zhu, Mantong;Zhu, Mantong;Liu, Chang;Liu, Guangfeng;Zhang, Hongsai;Wang, Xing;Wang, Ying;Luo, Yongqi;Jiang, Jingzhe

作者机构：

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.5； 五年影响因子：2.2 ）

ISSN： 0304-8608

年卷期： 2025 年 170 卷 8 期

页码：

收录情况： SCI

摘要： A Providencia strain was isolated from farm effluents and used as a host to isolate a bacteriophage named A06. A double-layer agar plaque assay was used to determine the optimal multiplicity of infection (MOI), construct a one-step growth curve, evaluate the temperature and pH stability of the phage, and assess its in vitro antibacterial activity and lysis efficiency. Whole-genome sequencing, functional annotation, and comparative genomics analysis were used to analyze its biological features and determine its taxonomic position. Bacteriophage A06 has a circular DNA genome with 58 open reading frames (ORFs). The optimal MOI was determined to be 100, and a one-step growth curve showed the latent period to be 40 minutes and the lysis period to range from 40 to 90 minutes. Bacteriophage A06 exhibited a high degree of thermal stability and pH tolerance, and genomic analysis indicated that it represents a new species within the genus Kakivirus of the family Autoscriptoviridae.

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