The synergy of thanatin and cathelicidin-BF-15a3 combats Escherichia coli O157:H7

文献类型： 外文期刊

第一作者： Xia, Xuefeng

作者： Xia, Xuefeng;Song, Shiying;Zhang, Shuangyu;Wang, Wei;Zhou, Junming;Fan, Baochao;Li, Li;Li, Bin;Zhang, Xuehan;Xia, Xuefeng;Fan, Baochao;Li, Li;Li, Bin;Zhang, Xuehan;Song, Shiying;Dong, Hailong;Li, Bin;Zhang, Xuehan;Luo, Chuping;Li, Bin;Wang, Wei;Zhou, Junming;Fan, Baochao;Li, Li;Li, Bin;Zhang, Xuehan;Xia, Xuefeng;Song, Shiying;Zhang, Shuangyu;Wang, Wei;Zhou, Junming;Fan, Baochao;Li, Li;Li, Bin;Zhang, Xuehan;Li, Bin;Zhang, Xuehan

作者机构：

关键词： Antimicrobial peptides; Combination use; Food safety; Gram-negative bacteria; Membrane integrity; Vegetables

期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.4； 五年影响因子：5.5 ）

ISSN： 0168-1605

年卷期： 2023 年 386 卷

页码：

收录情况： SCI

摘要： Escherichia coli O157:H7 is a pathogen that commonly causes foodborne illness and represents a health hazard to consumers. The combined use of synergistic antimicrobial peptides (AMPs) is a promising way to improve the microbiological safety of foods. In this study, we detected the synergistic interactions between thanatin and BF -15a3 to reduce their usage and obtain more efficient antibacterial activity. The minimal inhibitory concentra-tions (MICs) of thanatin and BF-15a3 against 49 E. coli O157:H7 strains were ranged from 2 to 8 mu g/mL and 4-32 mu g/mL, showed a general inhibitory effect on E. coli O157:H7 strains, respectively, even multidrug-resistant strains. Their fractional inhibitory concentration index (FICI) was 0.375, which suggested that their combina-tion presented synergistic antibacterial effect against E. coli O157:H7. The killing kinetic curves indicated that the 0.25 x MIC combination had equivalent bactericidal effects to 1 x MIC thanatin or BF-15a3. When AMP combinations were used to treat eukaryotic cells to evaluate the hemolytic characteristics against rabbit eryth-rocytes and cytotoxicity against human embryonic kidney 293T (HEK-293T) cells and intestinal porcine enter-ocyte J2 (IPEC-J2) cells, no magnified adverse effects were observed, exhibiting higher specificity to bacteria and lower toxicity to eukaryotic cells. Compared with bacteriostasis of thanatin or BF-15a3 alone, the proportion of membrane-damaged bacteria treated with the synergetic combination did not appear a significant rise, inter-estingly the Zeta potential of them greatly decreased and their cell membrane permeability significantly increased. Besides, more release of ions and cytoplasm were detected, confirming a more severe loss of mem-brane integrity. These results suggested that the synergistic action mode of thanatin and BF-15a3 is likely attributed to damage aggravation to E. coli membrane. When applying in fresh-cut lettuce and cucumber, their combination allowed for 2.5 log CFU/piece reductions of E. coli O157:H7 in 24 h. In conclusion, the combination of thanatin and BF-15a3 showed excellent synthetic efficacy to kill E. coli O157:H7 in vitro under lower MICs than single use of them.

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