PLAG1 g.8795C > T Mutation Regulates Early Body Weight in Hu Sheep by Weakening miR-139 Binding

文献类型： 外文期刊

第一作者： Wang, Yue

作者： Wang, Yue;Li, Yin-xia;Zhang, Jun;Qian, Yong;Meng, Chun-hua;Zhong, Ji-feng;Cao, Shao-xian;Wang, Yue;Li, Yin-xia;Zhang, Jun;Qian, Yong;Meng, Chun-hua;Zhong, Ji-feng;Cao, Shao-xian;Wang, Yue;Li, Yin-xia;Zhang, Jun;Qian, Yong;Meng, Chun-hua;Zhong, Ji-feng;Cao, Shao-xian

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关键词： birth weight; Hu sheep; miR-139; PLAG1; SNPs; weaning weight

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 2 期

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收录情况： SCI

摘要： Sheep birth and weaning weights indicate their growth and survival. Thus, identifying molecular genetic markers for early body weight is important in sheep breeding. Pleomorphic adenoma gene 1 (PLAG1) is important for regulating birth weight and body length in mammals; however, its relationship with sheep body weight remains unknown. Here, the 3 '-untranslated region (3 '-UTR) of the Hu sheep PLAG1 gene was cloned, single nucleotide polymorphisms (SNPs) were screened, genotype-early body weight relationships were analyzed, and the possible molecular mechanism was explored. PLAG1 3 '-UTR sequences with five forms of base sequences plus poly(A) tails were detected in Hu sheep and the g.8795C>T mutation was identified. Luciferase reporter assay indicated that the g.8795C>T mutation influenced PLAG1 post-transcriptional activity. miRBase prediction showed that the g.8795C>T mutation was located in the miR-139 seed sequence binding region, and miR-139 overexpression significantly decreased both PLAG1-CC and PLAG1-TT activities. Moreover, the luciferase activity of PLAG1-CC was significantly lower than that of the PLAG1-TT, but miR-139 inhibition substantially increased both PLAG1-CC and PLAG1-TT luciferase activities, suggesting that PLAG1 is the target gene of miR-139. Thus, the g.8795C>T mutation upregulates PLAG1 expression by weakening its binding with miR-139, promoting PLAG1 expression, and increasing Hu sheep birth and weaning weights.

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