An efficient Agrobacterium tumefaciens-mediated transformation system of Taraxacum kok-saghyz Rodin (Russian dandelion)
文献类型： 外文期刊
第一作者： Peng, Cunzhi
作者： Peng, Cunzhi;Chang, Lili;Wang, Dan;Zheng, Xingmei;Tong, Zheng;Peng, Cunzhi;Chang, Lili;Wang, Dan;Zheng, Xingmei;Tong, Zheng;Xie, Quanliang;Xu, Bingqiang
作者机构：
关键词： Taraxacum kok-saghyz Rodin; Genetic transformation system; Rubber elongation factor; Rubber-yielding plant
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.6； 五年影响因子：2.7 ）
ISSN： 1054-5476
年卷期： 2023 年 59 卷 3 期
页码：
收录情况： SCI
摘要： Taraxacum kok-saghyz Rodin (TK) is an excellent alternative to Hevea brasiliensis because of its high yield of latex, wide growth range, and short life cycle, and it is also an ideal plant for natural rubber research through genetic transformation. This study aimed to report an efficient Agrobacterium tumefaciens-mediated transformation system of TK using leaves as explants. A TK genotype, named 12-16, that is suitable for tissue culture was obtained from a wild germplasm repository in Xinjiang. The multiplication rate of TK genotype 12-16 reached 3.72 adventitious shoots per explant through cultivation on the shoot induction medium of Murashige-Skoog (MS) nutrient medium supplemented with 1.5 mg L-1 6-benzylaminopurine and 0.1 mg L-1 naphthaleneacetic acid. Moreover, the average length of adventitious shoots was 3.07 +/- 0.78 cm after 60 d in culture. Based on the transformation system, two genes of rubber elongation factor 138 (REF138) and rubber elongation factor 258 (REF258), driven under an enhanced 35S promoter, were transformed into the genotype 12-16 through Agrobacterium tumefaciens GV3101 mediated genetic transformation. The transformation process from leaf explants to hygromycin-resistant seedlings lasted approximately 3 to 4 mo, and the transformation efficiency reached 23.12 to 24.05%. This study established a highly efficient transformation system based on the TK genotype 12-16 and provided an important technical support for rubber biosynthesis research.
分类号：
- 相关文献
作者其他论文 更多>>
-
R2R3-type LoMYB21 affects jasmonate-regulated development and dehiscence of anthers in lily (Lilium oriental hybrids)
作者：Tong, Zheng;Tong, Zheng;Tong, Zheng;Dong, Tingting;Li, Qiuhua;Wang, Rui;He, Junna;Hong, Bo;Li, Qiuhua
关键词：Lilium; LoMYB21; Anther dehiscence; Jasmonate acid
-
The disease quantification analysis of cotton Verticillium wilt using the two methods of disease index and fungal biomass present high consistency
作者：Zhu, He;Li, Yue;Wang, Zi-sheng;Ji, Xiao-bin;Zhang, Dan -dan;Chen, Jie-yin;Dai, Xiao-feng;Wang, Dan;Zhang, Dan -dan;Chen, Jie-yin;Dai, Xiao-feng;Wang, Dan
关键词：Verticillium dahliae; disease grade classification; qPCR; correlation coefficient
-
TIP aquaporins in Cyperus esculentus: genome-wide identification, expression profiles, subcellular localizations, and interaction patterns
作者：Zou, Zhi;Zheng, Yujiao;Chang, Lili;Zou, Liangping;Zhao, Yongguo;Zhang, Li;Zhao, Yongguo;Min, Yi
关键词：Tigernut; Tonoplast intrinsic protein; Synteny analysis; Expression divergence; Subcellular localization; Protein interaction
-
The potential of Burkholderia gladioli KRS027 in plant growth promotion and biocontrol against Verticillium dahliae revealed by dual transcriptome of pathogen and host
作者：Wang, Dan;Lin, Haiping;Shan, Yujia;Song, Jian;Zhang, Dan-Dan;Dai, Xiao-Feng;Chen, Jie-Yin;Zhang, Dan-Dan;Dai, Xiao-Feng;Chen, Jie-Yin;Han, Dongfei;Han, Dongfei
关键词：Verticillium dahliae; Biological control; Burkholderia gladioli; Plant growth promotion; Transcriptome
-
Evolution of microbial community and the volatilome of fresh-cut chili pepper during storage under different temperature conditions: Correlation of microbiota and volatile organic compounds
作者：Li, Zudi;Zhao, Xiaoyan;Li, Zudi;Zhao, Wenting;Wang, Pan;Zhao, Shuang;Wang, Dan;Zhao, Xiaoyan
关键词：Fresh-cut chili pepper; Bacterial community; Fungal community; Microbial spoilage; VOCs; E-nose
-
Plant Growth Regulators Enhance Maize (Zea mays L.) Yield under High Density by Optimizing Canopy Structure and Delaying Leaf Senescence
作者：Xu, Tong;Wang, Dan;Si, Yu;Kong, Yuanyuan;Shao, Xiwen;Geng, Yanqiu;Lv, Yanjie;Wang, Yongjun;Lv, Yanjie;Wang, Yongjun
关键词：maize; plant density; plant growth regulator; canopy; pollen density; yield
-
A sensitive intelligent point-of-care test method for tert-butylhydroquinone in edible oil via a test strip with a smartphone
作者：Wang, Dan;Wu, Wenqin;Zhang, Zhaowei;Wang, Dan;Zhang, Le;Li, Wangwang;Tian, Zhi-Quan;Wang, Dan;Sun, Yuanlong;Tian, Zhi-Quan;Zeng, Jing;Wang, Shenling;Zhang, Zhaowei
关键词：Tert-butylhydroquinone; ZnCdSe/ZnS quantum dot; Intelligent; Point-of-care testing; Edible oil; Food safety
