m6A modification associated with YTHDF1 is involved in Japanese encephalitis virus infection

文献类型： 外文期刊

第一作者： Li, Xiao-han

作者： Li, Xiao-han;Chen, Jing;Ou, Yu-da;Hu, Jia-huan;Sun, Rui-cong;Lv, Ying-jun;Zhou, Bin;Zhong, Xiang;Wei, Jian-chao;Go, Yun Young

作者机构：

关键词： Japanese encephalitis virus; N6-methyladenosine; YTHDF1; ISGs; Antiviral

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）

ISSN： 0378-1135

年卷期： 2023 年 287 卷

页码：

收录情况： SCI

摘要： N6-methyladenosine (m6A), the most common modification in mammalian mRNA and viral RNA, regulates mRNA structure, stability, translation, and nuclear export. The Japanese encephalitis virus (JEV) is a mosquitoborne flavivirus causing severe neurologic disease in humans. To date, the role of m6A modification in JEV infection remains unclear. Herein, we aimed to determine the impact of m6A methylation modification on JEV replication in vitro and in vivo. Our results demonstrated that the overexpression of the m6A reader protein YTHDF1 in vitro significantly inhibits JEV proliferation. Additionally, YTHDF1 negatively regulates JEV proliferation in YTHDF1 knockdown cells and YTHDF1 knockout mice. MeRIP-seq analysis indicated that YTHDF1 interacts with several interferon-stimulated genes (ISGs), especially in IFIT3. Overall, our data showed that YTHDF1 played a vital role in inhibiting JEV replication. These findings bring novel insights into the specific mechanisms involved in the innate immune response to infection with JEV. They can be used in the development of novel therapeutics for controlling JEV infection.

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