Transcriptome profiling of Microbacterium resistens MZT7 reveals mechanisms of 17?-estradiol response and biotransformation

文献类型： 外文期刊

第一作者： Hao, Peng

作者： Hao, Peng;Lv, Zongshuo;Wu, Sicheng;Zhang, Xiqing;Gao, Yunhang;Gou, Changlong;Wang, Lixia;Zhu, Yanbin;Basang, Wangdui

作者机构：

关键词： 17?-estradiol; Microbacterium resistens MZT7; Microbial biodegradation; Intracellular enzymes; Transcriptome analysis; ABC transporters

期刊名称：ENVIRONMENTAL RESEARCH （ 影响因子：8.3； 五年影响因子：8.2 ）

ISSN： 0013-9351

年卷期： 2023 年 217 卷

页码：

收录情况： SCI

摘要： 17 beta-estradiol (E2) pollution has attracted much attention, and the existence of E2 poses certain risks to the environment and human health. However, the mechanism of microbial degradation of E2 remains unclear. In this study, the location of E2-degrading enzymes was investigated, and transcriptome analysis of Microbacterium resistens MZT7 (M. resistens MZT7) exposed to E2. The degradation of E2 by M. resistens MZT7 was via the biological action of E2-induced intracellular enzymes. With the RNA sequencing, we found 1109 differentially expressed genes (DEGs). Among them, 773 genes were up-regulated and 336 genes were down-regulated. The results of the RNA sequencing indicated the DEGs were related to transport, metabolism, and stress response. Genes for transport, transmembrane transport, oxidoreductase activity, ATPase activity, transporter activity and quorum sensing were up-regulated. Genes for the tricarboxylic acid cycle, ribosome, oxidative phosphorylation and carbon metabolism were down-regulated. In addition, heterologous expression of one enzymes efficiently degraded E2. These findings provide some new insights into the molecular mechanism of biotransformation of E2 by M. resistens MZT7.

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