Intestinal Tissue, Digestive Enzyme, and Antioxidant Enzyme Activities in the Early Development Stage of Endangered Brachymystax tsinlingensis

文献类型： 外文期刊

第一作者： Song, Rongqun

作者： Song, Rongqun;Wang, Zhenlu;Guo, Xingchen;Wang, Yizhou;Zhang, Lin;Shao, Jian;Song, Rongqun;Wang, Zhenlu;Guo, Xingchen;Wang, Yizhou;Zhang, Lin;Shao, Jian;Song, Rongqun;Wang, Zhenlu;Shao, Jian;Lin, Shaoqing;Ye, Huan

作者机构：

关键词： Brachymystax tsinlingensis; early development; intestinal tissue structure; digestive enzymes; antioxidant enzymes

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 20 期

页码：

收录情况： SCI

摘要： This work explores the digestive system characteristics of Brachymystax tsinlingensis during early developmental stages and aims to solve the problem of high lethality of fry during the transgression period, which is crucial for the artificial propagation and population conservation of endangered fishes. This study was carried out on intestinal tissue, digestive enzymes, and antioxidant enzyme activities in the early development stage of Brachymystax tsinlingensis. Ten random samples during endogenous nutrition (7, 10, and 11 days after hatching), mixed nutrition (13 and 19 DAH), and exogenous nutrition (31, 33, 39, 45, and 73 DAH) were collected by histological and biochemical analysis methods. The results showed that the intestine of Brachymystax tsinlingensis already has four layers initially at 7 DAH, and the intestinal gland tissue is evident at 73 DAH. The contents of total protein (TP) and the activities of lipase (LPS) and trypsin (TPS) were maximal at 39 DAH, and the activities were 3.20 +/- 0.26 mg/mL, 2.52 +/- 0.69 U/g, and 2717.45 +/- 295.26 U/mg, respectively. Catalase (CAT) and glutathione peroxidase (GSH-PX) activities both showed the lowest values at 39 DAH, which were 0.57 +/- 0.11 U/mg and 3.35 +/- 0.94 U/mg, respectively. The activity of amylase (AMS) and the content of malonaldehyde (MDA) increased, and the highest values were reached at 45 DAH (1.32 +/- 0.41 U/mg) and 73 DAH (1.29 +/- 0.43 nmoL/mg), respectively. Superoxide dismutase (SOD) and GSH-PX activities both showed a peak value at 7 DAH (126.58 +/- 20.13 U/mg and 6.47 +/- 1.86 U/mg). Overall, the changes in intestinal tissue, digestive enzymes, and antioxidant enzyme activities at 39 DAH of Brachymystax tsinlingensis are inseparable from different vegetative stages during the developmental period, and these results can provide a reference for the proliferation and cultivation of Brachymystax tsinlingensis resources.

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