A transcriptional complex of FtMYB102 and FtbHLH4 coordinately regulates the accumulation of rutin in Fagopyrum tataricum

文献类型： 外文期刊

第一作者： Mi, Yaolei

作者： Mi, Yaolei;Zhang, Dong;Mi, Yaolei;Li, Yu;Qian, Guangtao;Meng, Xiangxiao;Liu, Tingxia;Yang, Wei;Shi, Yuhua;Chen, Shilin;Sun, Wei;Li, Yu;Vanhaelewyn, Lucas;Vanhaelewyn, Lucas;Ma, Pengda;Tul-Wahab, Atia;Viczian, Andras

作者机构：

关键词： Tartary buckwheat ( Fagopyrum tataricum ); Secondary metabolism; Rutin; Transcription factors; Transcriptional regulation; Medicinal plants

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2023 年 194 卷

页码：

收录情况： SCI

摘要： Tartary buckwheat is rich in flavonoids, which not only play an important role in the plant-environment interaction, but are also beneficial to human health. Rutin is a therapeutic flavonol which is massively accumulated in Tartary buckwheat. It has been demonstrated that transcription factors control rutin biosynthesis. However, the transcriptional regulatory network of rutin is not fully clear. In this study, through transcriptome and target metabolomics, we validated the role of FtMYB102 and FtbHLH4 TFs at the different developmental stages of Tartary buckwheat. The elevated accumulation of rutin in the sprout appears to be closely associated with the expression of FtMYB102 and FtbHLH4. Yeast two-hybrid, transient luciferase activity and coimmunoprecipitation demonstrated that FtMYB102 and FtbHLH4 can interact and form a transcriptional complex. Moreover, yeast one-hybrid showed that both FtMYB102 and FtbHLH4 directly bind to the promoter of chalcone isomerase (CHI), and they can coordinately induce CHI expression as shown by transient luciferase activity assay. Finally, we transferred FtMYB102 and FtbHLH4 into the hairy roots of Tartary buckwheat and found that they both can promote the accumulation of rutin. Our results indicate that FtMYB102 and FtbHLH4 can form a transcriptional complex by inducing CHI expression to coordinately promote the accumulation of rutin.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Impact of residual antibiotics on microbial decomposition of livestock manures in Eutric Regosol: Implications for sustainable nutrient recycling and soil carbon sequestration

    作者：Fang, Linfa;Lakshmanan, Prakash;Su, Xiaoxuan;Shi, Yujia;Chen, Zheng;Zhang, Yu;Xiao, Ran;Chen, Xinping;Lakshmanan, Prakash;Wu, Junxi;Lakshmanan, Prakash;Lakshmanan, Prakash;Sun, Wei

    关键词：Residual antibiotics; Livestock manure decomposition; Microbial community; Co -occurrence network; Enzyme activities

  • Extreme drought impacts have been underestimated in grasslands and shrublands globally

    作者：Smith, Melinda D.;Knapp, Alan K.;Ohlert, Timothy;Smith, Melinda D.;Knapp, Alan K.;Wilkins, Kate D.;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Wilfahrt, Peter;Anderson, Maggie;Borer, Elizabeth T.;Isbell, Forest;Seabloom, Eric W.;Collins, Scott L.;Sala, Osvaldo E.;Jordan, Samuel;Dukes, Jeffrey S.;Phillips, Richard P.;Beier, Claus;Yahdjian, Laura;Biancari, Lucio;Bondaruk, Viviana Florencia;Onatibia, Gaston R.;Pineiro-Guerra, Juan Manuel;Gherardi, Laureano A.;Larsen, Klaus Steenberg;Fraser, Lauchlan H.;Jentsch, Anke;Niu, Yujie;Loik, Michael E.;Maestre, Fernando T.;Valdecantos, Alejandro;Maestre, Fernando T.;Gozalo, Beatriz;Maturano-Ruiz, Adrian;Valdecantos, Alejandro;Power, Sally A.;Chieppa, Jeff;Nielsen, Uffe N.;Yu, Qiang;Felton, Andrew J.;Munson, Seth M.;Luo, Yiqi;Bauerle, Taryn;Abdoli, Hamed;Abedi, Mehdi;Bahalkeh, Khadijeh;Alados, Concepcion L.;Castillo-Garcia, Miguel;Pueyo, Yolanda;Alberti, Juan;Bruschetti, Carlos Martin;Cardoni, Augusto;Daleo, Pedro;Iribarne, Oscar;Alon, Moshe;Cohen, Ofer;Sternberg, Marcelo;An, Hui;Anacker, Brian;Auge, Harald;Auge, Harald;Eisenhauer, Nico;Hoss, Daniela;Roscher, Christiane;Sunnemann, Marie;Bachle, Seton;Bachle, Seton;Bahn, Michael;Ingrisch, Johannes;Batbaatar, Amgaa;Cahill, James F., Jr.;Batbaatar, Amgaa;Bork, Edward W.;Carlyle, Cameron N.;Behn, Kai;Beil, Ilka;Kreyling, Juergen;Malyshev, Andrey V.;Blindow, Irmgard;Dahlke, Sven;Byrne, Kerry M.;Calvo, Dianela A.;Leder, Cintia V.;Peter, Guadalupe;Carbognani, Michele;Forte, T'ai G. W.;Petraglia, Alessandro;Chang, Scott X.;Cianciaruso, Marcus V.;Guidoni-Martins, Karlo G.;Nobre, Paola;Cordeiro, Amanda L.;Cusack, Daniela F.;Dietterich, Lee H.;D'Antonio, Carla M.;Dietterich, Lee H.;Doherty, Tim S.;Greenville, Aaron C.;Wardle, Glenda M.;Dubbert, Maren;Ebeling, Anne;Eisenhauer, Nico;Fischer, Felicia M.;Hoss, Daniela;Sunnemann, Marie;Hoss, Daniela;Pillar, Valerio D.;Fischer, Felicia M.;Gebauer, Tobias;Anacker, Brian;Haugum, Siri Vatso;Vandvik, Vigdis;Hautier, Yann;Hefting, Mariet;Henry, Hugh A. L.;Johnson, Yari;Kelly, Eugene F.;Kimmel, Kaitlin;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kubert, Angelika;Werner, Christiane;Lamb, Eric G.;Larson, Julie;O'Connor, Rory C.;Lawson, Jason;Plowes, Robert M.;Linstadter, Anja;Orban, Ildiko;Liu, Jielin;Liu, Shirong;Longo, Grisel;Loydi, Alejandro;Rodriguez, Dana Aylen;Scarfo, Maria Cecilia;Luan, Junwei;Wang, Yi;Luan, Junwei;Wang, Yi;Lubbe, Frederick Curtis;Wiehl, Georg;Mackie-Haas, Kathleen;Stampfi, Andreas;Merchant, Thomas;Metcalfe, Daniel B.;Mori, Akira S.;Mori, Akira S.;Mudongo, Edwin;Newman, Gregory S.;Souza, Lara;Nimmo, Dale;Ogaya, Roma;Penuelas, Josep;Osborne, Brooke;Otfinowski, Rafael;Partel, Meelis;Pineiro-Guerra, Juan Manuel;Peri, Pablo L.;Picon-Cochard, Catherine;Pineiro-Guerra, Juan Manuel;Ploughe, Laura W.;Portales-Reyes, Cristy;Reed, Sasha C.;Ritchie, Euan G.;Roscher, Christiane;Sanchez, Ana M.;Santo, Braulio A.;Shi, Baoku;Sun, Wei;Souza, Lara;Standish, Rachel J.;Zeiter, Michaela;Stampfi, Andreas;Zeiter, Michaela;Standish, Rachel J.;Tedder, Michelle;Thorvaldsen, Pal;Tian, Dashuan;Zhu, Juntao;Zong, Ning;Tielborger, Katja;van den Brink, Liesbeth;Vankoughnett, Mathew R.;Velle, Liv Guri;Wang, Changhui;Wei, Cunzheng;Williams, Jennifer L.;Wolf, Amelia A.;Zhang, Fawei;Zuo, Xiaoan;Zuo, Xiaoan;Gebauer, Tobias;Ploughe, Laura W.

    关键词：climate extreme; Drought-Net; International Drought Experiment; productivity

  • Circulating exosome-mediated AMPKα-SIRT1 pathway regulates lipid metabolism disorders in calf hepatocytes

    作者：Zhang, Daoliang;Liu, Chang;Huang, Yingying;Tai, Wenjun;Feng, Shibin;Wang, Xichun;Zhao, Chang;Li, Yu;Ding, Hongyan

    关键词：Dairy cow ketosis; Circulating exosome; Lipid metabolism disorder; AMPK alpha-SIRT1 pathway

  • Seedling root system adaptation to water availability during maize domestication and global expansion

    作者：Yu, Peng;He, Xiaoming;Wang, Danning;Li, Hongjie;Marcon, Caroline;Hochholdinger, Frank;Yu, Peng;He, Xiaoming;Wang, Danning;Li, Hongjie;Li, Chunhui;Li, Yu;Wang, Tianyu;Li, Meng;Perez-Limon, Sergio;Sawers, Ruairidh J. H.;Chen, Xinping;Chen, Xinping;Delgado-Baquerizo, Manuel;Delgado-Baquerizo, Manuel;Koller, Robert;Metzner, Ralf;van Dusschoten, Dagmar;Pflugfelder, Daniel;Borisjuk, Ljudmilla;Plutenko, Iaroslav;Mahon, Audrey;Resende, Marcio F. R.;Salvi, Silvio;Akale, Asegidew;Abdalla, Mohanned;Ahmed, Mutez Ali;Bauer, Felix Maximilian;Schnepf, Andrea;Lobet, Guillaume;Lobet, Guillaume;Heymans, Adrien;Suresh, Kiran;Schreiber, Lukas;McLaughlin, Chloee M.;Li, Chunjian;Mayer, Manfred;Schoen, Chris-Carolin;Bernau, Vivian;Bernau, Vivian;von Wiren, Nicolaus

    关键词：

  • miR-329b-5p Affects Sheep Intestinal Epithelial Cells against Escherichia coli F17 Infection

    作者：Xu, Yeling;Chen, Weihao;Sun, Wei;Yang, Huiguo;Song, Zhenghai;Wang, Yeqing;Su, Rui;Mwacharo, Joram M.;Lv, Xiaoyang;Sun, Wei;Lv, Xiaoyang;Sun, Wei

    关键词：Escherichia coli F17; micro RNA; sheep diarrhea; intestinal epithelial cells

  • Identification of a linear B-cell epitope on the "puff" loop of the Senecavirus A VP2 protein involved in receptor binding

    作者：Zhou, Hanrong;Sun, Mingxia;Su, Shibo;Meng, Liang;Shi, Xinqi;Li, Xin;Wang, Haiwei;Ma, Hongwei;Cai, Xuehui;Tang, Yan-Dong;An, Tongqing;Meng, Fandan;Yang, Wei;Yang, Lan;Ma, Hongwei;Cai, Xuehui;An, Tongqing

    关键词：Senecavirus A (SVA); B-cell epitopes (BCEs); monoclonal antibodies (mAbs); VP2 protein; receptor binding

  • Reef effect of vertical relief on the shelter-seeking and foraging processes of juvenile fat greenling ( Hexagrammos otakii ) in a mesocosm experiment

    作者：Zhang, Yue;Sun, Tao;Yang, Wei;Wang, Xiaoling;Sun, Qianzhao;Lin, Haiying;Ding, Gang;Yu, Daode;Li, Bo;Li, Shuhan

    关键词：Artificial reef; Vertical relief; Hexagrammos otakii; Shelter -seeking; Forage; Structural design

微信小程序