CRISPR/Cas-SERS Sensing Platforms: A Frontier Technology for Next-Generation Fast, Low-Cost, Ultra-Micro Biosample Detection

文献类型： 外文期刊

第一作者： Bao, Chengxin

作者： Bao, Chengxin;Xu, Shuping;Liu, Xiangguo;Liang, Chongyang;Xu, Shuping

作者机构：

关键词： CRISPR/Cas detection; nucleic acid analysis; SERS detection; sgRNA

期刊名称：JOURNAL OF RAMAN SPECTROSCOPY （ 影响因子：1.9； 五年影响因子：2.5 ）

ISSN： 0377-0486

年卷期： 2025 年

页码：

收录情况： SCI

摘要： Researchers have long been interested in nucleic acid detection technology. Surface-enhanced Raman spectroscopy (SERS) is distinguished by its high sensitivity, minimal sample volume, resistance to fluorescence interference, cost-effectiveness, and rapidity compared to conventional nucleic acid analysis methods. The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas), a novel gene editing tool, has garnered significant interest in nucleic acid analysis due to its precise identification and isothermal advantages. Integrating the CRISPR/Cas system's specific identification capabilities with the high-sensitivity fingerprinting properties of SERS offers a sensitive, ultra-low volume, rapid, and straightforward method for detecting new nucleic acid modalities. This review delineates the components and characteristics of the CRISPR/Cas system, encompassing three Cas proteins (Cas9, Cas12, and Cas13) and detection technologies derived from CRISPR/Cas, namely, high-sensitivity enzymatic reporter unlocking (SHERLOCK) and DNA endonuclease-targeted CRISPR trans reporters (DETECTR). Advancements in SERS and CRISPR/Cas-SERS-based nucleic acid assays were emphasized, encompassing traditional SERS and CRISPR/Cas-SERS-based nucleic acid and non-nucleic acid tests. Examples encompass microfluidics/microdroplet-based CRISPR/Cas-SERS and non-amplified detection based on CRISPR/Cas-SERS, and so on.

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