Ultrasound-assisted preparation of antioxidant peptides in flaxseed meal: Purification, characterization and molecular docking analysis

文献类型： 外文期刊

第一作者： Wei, Kejun

作者： Wei, Kejun;Wan, Yilai;Wei, Changqing;Liu, Wenyu;Leng, Yuanyuan;Xu, Minghui;Li, Yazhuan;Chen, Zhanglian;Wang, Jing;Luo, Huixin;Wei, Kejun;Wan, Yilai;Wei, Changqing;Liu, Wenyu;Leng, Yuanyuan;Xu, Minghui;Li, Yazhuan;Chen, Zhanglian;Wang, Jing;Luo, Huixin;Wei, Kejun;Wan, Yilai;Wei, Changqing;Liu, Wenyu;Leng, Yuanyuan;Xu, Minghui;Li, Yazhuan;Chen, Zhanglian;Wang, Jing;Luo, Huixin;Wu, Hongbin

作者机构：

关键词： Flaxseed meal; Ultrasound-assisted enzymatic hydrolysis; Antioxidant peptide; In silico analysis; Molecular docking; Myeloperoxidase

期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）

ISSN： 0308-8146

年卷期： 2025 年 487 卷

页码：

收录情况： SCI

摘要： Flaxseed meal is recognized as a rich source of high-quality protein, and this study aims to explore its potential as a source of bioactive peptides. Hydrolysates were prepared via ultrasound-assisted enzymatic hydrolysis. Enzymatic hydrolysates from hot-pressed flaxseed meal showed higher biological activity than those from coldpressed samples. Three antioxidant peptides, namely PFFWLHHT, HCLEFLSPRF, and ALTMPHNW, were identified and screened from the purified fractions of hot-pressed flaxseed meal using reversed-phase liquid chromatography-mass spectrometry (RPLC-MS) and in silico analysis. These peptides displayed scavenging activity against DPPH radicals (IC50 = 0.520, 1.190, and 1.036 mM) and hydroxyl radicals (IC50 = 4.909, 6.471, and 7.076 mM). Molecular docking studies indicated that three peptides could inhibit myeloperoxidase (MPO) activity by occupying the entrance of the active cavity, with their binding affinities measured at -12.2, -9.1 and - 11 kcal/mol, respectively. These findings could lay a foundation for extracting natural antioxidant peptides from flaxseed meals.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序