Cloning of Acyl-ACP thioesterase FatA from Arachis hypogaea L. and its expression in Escherichia coli
文献类型： 外文期刊
第一作者： Chen, G.
作者： Chen, G.;Peng, Z. Y.;Xuan, N.;Zhang, Y.;Bi, Y. P.;Chen, G.
作者机构：
关键词： Acyl-acyl carrier protein thioesterases; rapid amplification of cDNA ends; fatty acid; peanut
期刊名称：RESEARCH JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.233； 五年影响因子：0.252 ）
ISSN： 2278-4535
年卷期： 2012 年 7 卷 4 期
页码：
收录情况： SCI
摘要： In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3'-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and showed 50-70% identity with FatA from other plants. Real-time quantitative PCR analysis revealed that AhFatA was expressed in all tissues of A. hypogaea L., but most strongly in the immature seeds harvested at 60 days after pegging. Heterologous expression of AhFatA in Escherichia coli affected bacterial growth and changed the fatty acid profiles of the membrane lipid, resulting in directed accumulation towards palmitoleic acid and oleic acid. These results indicate that AhFatA is at least partially responsible for determining the high palmitoleic acid and oleic acid composition of E. coli.
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