Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis

文献类型： 外文期刊

第一作者： Liu, Jingyi

作者： Liu, Jingyi;Sheng, Fei;Giri, Bikash R.;Cheng, Guofeng;Liu, Jingyi;Li, Shun;Xia, Tianqi;Li, Xuxin;Wang, Xiaoxu

作者机构：

期刊名称：ISCIENCE （ 影响因子：5.8； 五年影响因子：5.8 ）

ISSN：

年卷期： 2023 年 26 卷 9 期

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收录情况： SCI

摘要： Timely diagnosis of Schistosoma infection, particularly in the early stage is crucial for identifying infected hosts and then taking effective control strategies. Here, metagenomic next-generation sequencingwas used to identify pathogen-specific circulating DNAs (cDNAs) in the sera/plasma of New Zealand rabbits infected with S. japonicum, and the identified cDNAs were validated by PCR and qPCR. Loop-mediated isothermal amplification (LAMP)-based CRISPR-Cas12a and recombinase polymerase amplification-based lateral flow strip (RPA-LF) methods combined with the newly identified cDNAwere developed to evaluate the potentials for diagnosing murine and human schistosomiasis. The results indicated that twenty-two cDNAs were identified. The developed LAMP-based CRISPR/Cas12a and RPA-LF methods showed a good potential for diagnosing murine or human schistosomiasis as early as 5 days of post-infection with 5 cercariae infection. In a word, S. japonicum specific cDNAs in circulation of infected hosts could be effective biomarkers for detecting Schistosoma infection particularly for early stages.

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