Dual-mode sensing of biomarkers based on nano 3D Cu-Flo center dot@AuNPs-electrocatalyzed oxidation of glucose inducing in-situ H2O2-generation system

文献类型： 外文期刊

第一作者： Guo, Jianping

作者： Guo, Jianping;Li, Shijie;Wang, Junping;Wang, Junying

作者机构：

关键词： Cu-Flo center dot nanosheet; H2O2-generation; Electrocatalysis; ECL-Biosensing

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：12.545； 五年影响因子：10.722 ）

ISSN： 0956-5663

年卷期： 2022 年 198 卷

页码：

收录情况： SCI

摘要： A bimodal 3D-electrochemiluminescence (ECL) analysis method was developed, which integrated simpleness, label-free, high-throughput and real time detection together. Firstly, a novel 3D copper-based nanosheet micro-material (Cu-Flo center dot NMs) coupled with gold nanoparticles/Cysteine (Cu-Flo center dot@AuNPs-Cys) was prepared to use as the versatile label for both colorimetric and ECL techniques. The 3D-Cu-Flo.@AuNPs-Cys having glucose oxidase-like activity could catalyze glucose to produce H2O2 in situ, which was further found to be capable of exhibiting a 30.95-fold higher ECL-intensity for luminol than bare glassy carbon electrodes (GCE). Taking advantages of the 3D-Cu-Flo.@AuNPs-Cys above, a colorimetric and ECL-channel sensor (GCE/3D-Cu-Flo center dot@AuNPs-Cys) were constructed simultaneously for glucose detection. The fabricated sensor displayed a wide linear range (Glucose: 0.001-50 mmol L-1, AFP: 2.25 x 10(-7)-225 ng mL(-1)), impressive low limit of detection (Glucose: 1.27 x 10(-7) mol L-1, AFP: 1.92 x 10(-8) ng mL(-1), S/N = 3) and acceptable recovery (Glucose: 94% similar to 104%, AFP: 96.04% similar to 102.29%) in practical sample. Furthermore, the biosensor showed ultrafast (0.5 min) analysis efficiency, high stability for 6 cyclic potential scans and satisfactory reproducibility for 7 repeated tests. These results demonstrated the proposed 3D dual-modal ECL-biosensor for biomarkers detection had a great potential in clinical diagnostics, promoting the application in biomedical researching and POCT.

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