Oviductal sperm storage in the Chinese pond turtle, Mauremys reevesii, depends on androgen-based promotion of the BCL 2 anti-apoptotic pathway

文献类型： 外文期刊

第一作者： Chen, Wei

作者： Chen, Wei;Wang, Yajie;Gong, Mengmeng;Gao, Hailin;Bu, Xingjiang;Xia, Xingquan;Nie, Liuwang;Zhou, Huaxing;Bu, Xingjiang;Bu, Xingjiang

作者机构：

关键词： androgen receptor; apoptosis; BAX; BCL 2; Mauremys reevesii; oviduct; reproductive tract; reptiles; sperm storage

期刊名称：REPRODUCTION FERTILITY AND DEVELOPMENT （ 影响因子：1.9； 五年影响因子：2.0 ）

ISSN： 1031-3613

年卷期： 2023 年 35 卷 6 期

页码：

收录情况： SCI

摘要： Context. Sperm storage is a complex and highly coordinated process that is regulated by a variety of factors. The BCL 2 protein family plays a key role in regulating apoptosis, and determines sperm survival. Aims. The objective of this study was to explore the correlation between sperm storage and the BCL 2 protein family in the oviduct of Mauremys reevesii. Methods. Hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) and real-time quantitative polymerase chain reaction (RT-qPCR) techniques were used to investigate three parts of the reproductive tract (isthmus, uterus and vagina) of mated and unmated female M. reevesii. Key results. Hematoxylin-eosin staining revealed many sperm stored in the oviduct. IHC showed positive immunostaining for the BCL 2 and BAX proteins in epithelial ciliated and glandular cells. RT-qPCR indicated that the mRNA expressions of anti-apoptotic genes (BCL 2, MCL 1, BCL-W, BCL-XL) and the androgen receptor (AR) were significantly higher in mated turtles than unmated turtles. However, the expression of pro-apoptotic genes (BAX, BAD, BID and CASPASE 3) showed the opposite relationship. Conclusions. These results suggest that sperm entering the oviduct can promote the synthesis of anti-apoptotic genes to protect themselves from various degradation factors. Implications. These findings will help researchers understand the mechanisms of sperm storage.

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