Comparative transcriptomic insight into orchestrating mode of dielectric barrier discharge cold plasma and lactate in synergistic inactivation and biofilm-suppression of Pichia manshurica

文献类型： 外文期刊

第一作者： Zhao, Nan

作者： Zhao, Nan;Mei, Yuan;Hou, Xinlei;Yang, Menglu;Li, Huajia;Liao, Qi;Ge, Lihong;Zhao, Jichun

作者机构：

关键词： Pichia manshurica; Inactivation; Biofilm; DBD; Lactate; Synergistic action mode

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.0； 五年影响因子：8.5 ）

ISSN： 0963-9969

年卷期： 2024 年 198 卷

页码：

收录情况： SCI

摘要： Pichia manshurica is a representative species of biofilm-forming yeasts which usually induces the spoilage of fermented food. This study aims to investigate the synergistic inactivating and anti-biofilm effect of dielectric barrier discharge cold plasma (DBD) and lactate on Pichia manshurica (P. manshurica) and the underlying mechanism by comparison of survival rate, growth curve, biofilm-forming capacity and transcriptome of P. manshurica treated with control (CK), lactate (LA), DBD, and combination of DBD and lactate (DBD-LA). Results showed that CK and LA hardly influenced the growth and biofilm formation of P. manshurica. DBD and DBD-LA reduced survival rate to 35 % and 10 % immediately after treatment, respectively. Also, with growth curve remaining plateau, DBD-LA completely inhibited the growth and biofilm formation of P. manshurica, while DBD moderately reduced the growth density and biofilm. Comparative transcriptomic analysis revealed that single DBD treatment intervened in the functions and pathways associated with DNA replication and cell adhesion (down-regulated expression of flocculation protein-related genes and up-regulated expression of beta-1,4D-glucan cellobiohydrolase-related genes). Lactate reinforced the inactivating and anti-biofilm effect of DBD by stimulating redox reaction and suppressing functions and pathways involving synthesis and metabolism of lipid and membrane, cation binding and organelle assembly. This study demonstrated the potential of synergistic combination of DBD and lactate in efficient control of biofilm-related spoilage of food by yeast.

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