Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds

文献类型： 外文期刊

第一作者： Zhu, Yingying

作者： Zhu, Yingying;Dun, Baoqing;Shi, Zhenxing;Wang, Yuanji;Wu, Li;Yao, Yang;Zhu, Yingying;Shi, Zhenxing

作者机构：

关键词： chickpea; polysaccharides; chemical structural; antioxidant; immunoregulatory

期刊名称：FRONTIERS IN NUTRITION （ 影响因子：6.59； 五年影响因子：6.873 ）

ISSN： 2296-861X

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： Two water-extractable polysaccharide fractions designated as CWP (7. 37 x 10(5) Da) and CWP-0.2 (1.58 x 10(4) Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds. The chemical structure of the two polysaccharides was characterized by various methods. Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56. Further structural characterization indicated that the main chain connection of CWP was -> (2-beta-d-Fruf-1) n ->, and the main chain connection of CWP-0.2 was explored as -> 2,4)-alpha-l-Rhap-(1 -> 3)-alpha-d-Galp-(1 -> with the branched chain of -> 2,4)-alpha-l-Rhap-(1 -> o-4. Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH center dot and ABTS center dot(+) as well as stimulating production of NO, IL-6, TNF-alpha and MCP-1 in RAW 264.7 macrophages. CWP-0.2 revealed significantly higher bioactivity than CWP.

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