Identification of the MADS-Box Gene Family and the Key Role of BrAGL27 in the Regulation of Flowering in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

文献类型： 外文期刊

第一作者： Gao, Xinyu

作者： Gao, Xinyu;Dai, Yun;Li, Xiangqianchen;Huang, Can;Zhang, Shifan;Li, Fei;Zhang, Hui;Li, Guoliang;Sun, Rifei;Zhang, Shujiang;Li, Yang;Song, Huanzhong;Zhang, Li;Chen, Zhendong

作者机构：

关键词： BrAGL27; Chinese cabbage; MADS-box gene family; vernalization

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 6 期

页码：

收录情况： SCI

摘要： Chinese cabbage (Brassica rapa L. ssp. pekinensis) is a key vegetable crop in Asia, but its commercial value is often reduced by premature flowering triggered by vernalization. The molecular mechanisms behind this process are not fully understood. MADS-box genes, as crucial transcriptional regulators, play vital roles in plant development, including flowering. In this study, 102 MADS-box genes were identified in Chinese cabbage through bioinformatics analyses, covering phylogeny, chromosomal localization, and gene structure. Real-time quantitative PCR and RNA-seq data analysis revealed that the expression level of AGL27 declined as vernalization time increased. To determine BrAGL27 ' s functions, we obtained BrAGL27-overexpressed (OE) Arabidopsis thaliana lines that showed significantly later flowering compared with the wild type (WT). The expression levels of flowering suppressor genes AtFLC and AtTEM1 were significantly high-regulated in the BrAGL27-OE lines compared to WT plants, while the expression levels of the floral genes AtSPL15, AtSOC1, AtFT, and AtAP3 were significantly lower in the BrAGL27-overexpressed lines than in the wild type. These findings enhance understanding of MADS-box genes in vernalization and flowering regulation, offering a basis for further research on bolting resistance and flowering control in Chinese cabbage.

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