Development and Utilization of Quantitative Detection for Probiotics Bacillus velezensis B31 with Potential Protection Against Tomato Fusarium Wilt

文献类型： 外文期刊

第一作者： Su, Zhenhe

作者： Su, Zhenhe;Gao, Yanan;Guo, Qinggang;Ma, Ping;Gao, Yanan;He, Yunzhuan;Dong, Lemeng

作者机构：

关键词： Bacillus velezensis; Unique orf3804; Strain-specific primers; Rhizosphere colonization; Biocontrol monitoring; Fusarium wilt biocontrol

期刊名称：PROBIOTICS AND ANTIMICROBIAL PROTEINS （ 影响因子：4.4； 五年影响因子：5.0 ）

ISSN： 1867-1306

年卷期： 2025 年

页码：

收录情况： SCI

摘要： The biocontrol efficacy of probiotics Bacillus velezensis B31 against fusarium wilt is contingent upon its successful colonization in the plant rhizosphere. This study aimed to develop a quantitative detection method for probiotics strain B31 and to monitor its rhizosphere dynamics. Utilizing comparative genomics, a specific gene in strain B31 was identified, which facilitated the design of precise primers and a Taqman probe. The resulting real-time PCR assay demonstrated high specificity to strain B31 and high sensitivity with detection limit of 28 copies/mu L of the genome. Comparative analysis with regular PCR methods revealed a strong correlation, yet highlighted the superior sensitivity and rapidity of the real-time PCR technique. The temporal colonization profile of strain B31, post root-inoculation, was charted using this assay. An initial rhizosphere population of 7.2 x 107 CFU g-1 was observed, which then tapered to 105 CFU g-1 over 14 days. These dynamics corresponded with the observed control effect, where a higher initial population correlated with enhanced disease suppression. This study underscores the potential of real-time PCR as a robust tool for biocontrol agents' monitoring, offering insights into the microbial interactions within the rhizosphere.

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