Integrated Transcriptomic and Metabolomic Analyses Reveal the Importance of the Terpenoid, Fatty Acid, and Flavonoid Pathways in Rice Cell Death and Defense

文献类型： 外文期刊

第一作者： Bai, Pengfei

作者： Bai, Pengfei;Liu, Yanfang;Choi, Naeyeoung;Wang, Guo-Liang;Liu, Yanfang;Gomes-Dias, Laisa;Combs-Giroir, Rachel;Lin, Yun;Blakeslee, Joshua J.;Gomes-Dias, Laisa;Lin, Yun;Blakeslee, Joshua J.;Gomes-Dias, Laisa;Combs-Giroir, Rachel;Dai, Shaoxing;Blakeslee, Joshua J.;Bernier, Matthew;Hatzakis, Emmanuel

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关键词： rice blast; cell death; metabolomics; transcriptomics; terpenoid; gibberellins

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 5 期

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收录情况： SCI

摘要： Lesion mimic mutants provide unique tools to investigate plant-pathogen interactions, often exhibiting hypersensitive responses in the absence of biotic or abiotic stresses. The overexpression of the S-domain receptor-like kinase gene, SPL11 cell-death suppressor 2 (SDS2), in rice leads to constitutive programmed cell death and enhanced resistance to fungal and bacterial pathogens. However, the mechanisms underlying this broad-spectrum resistance remain unclear. This study integrates transcriptomic and metabolomic analyses of the SDS2-ACT mutant to uncover gene expression and metabolic shifts associated with disease resistance. To identify SDS2-specific physiological changes related to pathogen resistance, leaf tissues from the SDS2-ACT mutant and the Kitkaake WT line were subjected to both transcriptomic and non-targeted metabolic profiling. Transcriptomic analyses identified 1497 differentially expressed genes (DEGs), including up-regulated genes involved in terpenoid and flavonoid biosynthesis, phytohormone signaling, and defense-related pathways (including pathogenesis-related [PR] genes). Metabolomic profiling revealed significant alterations in the accumulation of several compound classes, including putative: terpenoids, phenylpropanoids, phytohormones, fatty acids, and sugars. These changes are likely correlated with the observed cell death and resistance phenotypes in the SDS2-ACT mutant. This study provides an overall landscape of the transcriptomic and metabolomic alterations in a lesion mimic mutant, identifying candidate defense-related genes and metabolites for functional analysis in rice.

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