Novel Insights into the Control of Sitophilus zeamais in Grain by Chitosan-Nanoparticle-Contained dsSzCOX
文献类型： 外文期刊
第一作者： Lu, Xiao-Peng
作者： Lu, Xiao-Peng;Jiang, Lin-Lin;Li, Yue;Hou, Pu-Xing;Xu, Jia-Lu;Wu, Hua;Ma, Zhi-Qing;Zhou, Xiao-Yun;Wu, Hua;Ma, Zhi-Qing
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期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年 73 卷 10 期
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收录情况： SCI
摘要： Sitophilus zeamais is a highly destructive pest of stored grains worldwide. To develop novel RNA insecticides, the double-stranded RNA of S. zeamais cytochrome c oxidase (dsSzCOXs: dsSzCOXI, dsSzCOXII, and dsSzCOXIII) was successfully expressed in the pET28a-BL21(DE3)-RNaseIII- system and remarkably downregulated the expression level of SzCOXs in S. zeamais through injection or feeding methods, leading to high mortalities in both larvae and adults. Under their optimal expression conditions (isopropyl beta-d-thiogalactoside, 0.6 mM), the yields of dsSzCOXI (at 28 degrees C for 4 h), dsSzCOXII (at 37 degrees C for 6 h), and dsSzCOXIII (at 37 degrees C for 6 h) were 8.49, 5.80, and 6.04 mu g/mL respectively. The chitosan-based gene delivery system composed of dsSzCOXs (CS@dsSzCOXs) was established for the insecticidal effect of S. zeamais adults via mixing wheat seeds, with the mortality reaching 50% on day ten. Consequently, this nanoparticle-mediated gene delivery system is expected to be an effective approach for S. zeamais control.
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