Transcriptome analysis revealed alternative splicing landscape in response to gray-blight disease in tea plant

文献类型： 外文期刊

第一作者： Xu, Linlin

作者： Xu, Linlin;Deng, Jie;Wang, Hui;Zhang, Li;Luo, Lingyao;Xie, Hui;Liu, Shengrui;Huang, Songyan;Wei, Chaoling;Zhu, Junyan;Wang, Shuangshuang;Mi, Xiaozeng

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关键词： Alternative splicing; Camellia sinensis; Pestalotiopsis -like pathogen; Catechins

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 225 卷

页码：

收录情况： SCI

摘要： Alternative splicing (AS) is a post-transcriptional mechanism that plays crucial roles in plant development, as well as in mediating responses to biotic and abiotic stresses, by increasing the diversity of the transcriptome and proteome. However, our understanding of the functions of AS in tea plants ( Camellia sinensis) in response to pathogenic infections is limited. In this study, the characteristics of AS in tea plants infected with Pestalotiopsis- like pathogens at different time points were examined using RNA-sequencing (RNA-Seq). In total, 1768 differentially expressed AS genes (DASGs) were identified in the transcriptome of infected leaves, of which enriched in the flavonoid, anthocyanin biosynthesis pathway, and RNA splicing preocess. Notably, content of five different catechin monomers was significantly higher in pathogen-infected leaves. By constructing a co-expression network, numerous catechins-related genes were identified and characterized with a considerably higher frequency of AS events in infected leaves, which expression of those transcripts positively associated with catechins content. Both of CsDFR1-1 and its alternatively spliced transcripts ( CsDFR1-2 ) were collectively induced in leaves infected with gray-blight disease, which exhibited significantly positive correlation with epigallocatechin (ECG) and epicatechin (EC). Our study revealed the dynamic characteristics of AS in tea plants infected with gray-blight disease, providing novel insights into AS-mediated catechins biosynthesis involved in the defense against pathogen infection.

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