Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis

文献类型： 外文期刊

第一作者： Qin, Xing

作者： Qin, Xing;Su, Xiaoyun;Tu, Tao;Zhang, Jie;Wang, Xiaolu;Wang, Yaru;Wang, Yuan;Bai, Yingguo;Yao, Bin;Luo, Huiying;Huang, Huoqing

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关键词： dye-decolorizing peroxidase; mycotoxin; aflatoxin B-1; zearalenone; deoxynivalenol; biotransformation

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN：

年卷期： 2021 年 13 卷 6 期

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收录情况： SCI

摘要： The co-occurrence of multiple mycotoxins, including aflatoxin B-1 (AFB(1)), zearalenone (ZEN) and deoxynivalenol (DON), widely exists in cereal-based animal feed and food. At present, most reported mycotoxins degrading enzymes target only a certain type of mycotoxins. Therefore, it is of great significance for mining enzymes involved in the simultaneous degradation of different types of mycotoxins. In this study, a dye-decolorizing peroxidase-encoding gene BsDyP from Bacillus subtilis SCK6 was cloned and expressed in Escherichia coli BL21/pG-Tf2. The purified recombinant BsDyP was capable of oxidizing various substrates, including lignin phenolic model compounds 2,6-dimethylphenol and guaiacol, the substrate 2,2 '-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), anthraquinone dye reactive blue 19 and azo dye reactive black 5, as well as Mn2+. In addition, BsDyP could efficiently degrade different types of mycotoxins, including AFB(1), ZEN and DON, in presence of Mn2+. More important, the toxicities of their corresponding enzymatic degradation products AFB(1)-diol, 15-OH-ZEN and C15H18O8 were significantly lower than AFB(1), ZEN and DON. In summary, these results proved that BsDyP was a promising candidate for the simultaneous degradation of multiple mycotoxins in animal feed and food.

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