Interaction of a bacterial non-classically secreted RNase HI with a citrus B-Box zinc finger protein delays flowering in Arabidopsis thaliana and suppresses the expression of FLOWERING LOCUS T

文献类型： 外文期刊

第一作者： Du, Peixiu

作者： Du, Peixiu;Li, Weimin;Du, Peixiu;Du, Peixiu;Li, Weimin;Hu, Junxia;Gao, Xiaoyu;Yang, Wendi;Li, Weimin;Du, Meixia;Zou, Xiuping;Wang, Xuefeng;Zhang, Chao

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关键词： Candidatus Liberibacter asiaticus; Non-classically secreted protein; RNase HI; B-box zinc finger protein; Flowering; FLOWERING LOCUS T

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：6.7； 五年影响因子：7.1 ）

ISSN： 0944-5013

年卷期： 2024 年 278 卷

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收录情况： SCI

摘要： Ribonuclease HI (RNase HI) is well conserved across prokaryotes and eukaryotes, and has long been known to localize in the nucleic acid-containing cellular compartments for acting as an R-loop eraser but has never been determined to be a secreted protein. "Candidatus Liberibacter asiaticus" (CLas) is a fastidious alpha-proteobacterium that causes Huanglongbing (HLB), a devastating citrus disease often associated with flowering out of season. In this study, using the SecretomeP program coupled with an Escherichia coli-based alkaline phosphatase assay, we demonstrated that the CLas RNase HI (LasRNHI) was a non-classically secreted protein. Further experiments identified that LasRNHI could interact with a citrus B-box zinc finger protein CsBBX28 in the plant nucleolus. The in vitro assays indicated that CsBBX28 dramatically enhanced the R-loop-degrading activity of LasRNHI. Remarkably, co-expression of CsBBX28 and LasRNHI in Arabidopsis thaliana led to a much later flowering time than that of wild-type Arabidopsis, as well as that of the transgenic A. thaliana expressing only CsBBX28 or LasRNHI, and lastingly and significantly repressed transcription of FLOWERING LOCUS T (FT), a floral pathway integrator. Similarly, ectopic expression of LasRNHI in citrus greatly reduced the transcription level of FT. The data together disclosed the extracellular secretion of LasRNHI, and that LasRNHI physically interacted with CsBBX28 and served as a flowering repressor through suppressing the FT expression, suggesting a novel role of RNase HI in the bacteria interacting with the host plants.

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