Enantiomeric Separation and Circular Dichroism Detection of Metalaxyl Acid Metabolite by Chiral High Performance Liquid Chromatography

文献类型: 外文期刊

第一作者: Qiu, Jing

作者: Qiu, Jing;Wang, Qiuxia;Zhou, Zhiqiang;Yang, Shuming

作者机构:

关键词: Metalaxyl;Metabolite;Enantiomeric separation;Circular dichroism.

期刊名称:ASIAN JOURNAL OF CHEMISTRY ( 影响因子:0.355; 五年影响因子:0.293 )

ISSN:

年卷期:

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收录情况: SCI

摘要: The enantiomers of metalaxyl acid metabolite (MX-acid) were separated by HPLC using cellulose-tris-(3,5-dimethylphenyl-carbamate) (CDMPC) as chiral stationary phase. The effects of mobile phase composition,flow rate and column temperature on the resolution were investigated and good separation was achieved with n-hexane/2-propanol/trifluoro-acetic acid as mobile phase. The circular dichroisms detection showed that metalaxyl-acid has similar absorption behaviour to metalaxyl with CD signals reverse to optical rotation direction of S(+)- and R(-)-enantiomer and CD signals of metalaxyl intermediate enantiomers may be reversed with change of CD wavelength.

分类号: O6

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