McvR, a single domain response regulator regulates motility and virulence in the plant pathogen Xanthomonas campestris

文献类型： 外文期刊

第一作者： Li, Rui-Fang

作者： Li, Rui-Fang;Zhu, Gui-Ning;Xian, Xiao-Yong;Li, Rui-Fang;Ren, Pei-Dong;Liu, Qian-Qian;Yao, Jia-Li;Wu, Liu;Tang, Ji-Liang;Lu, Guang-Tao

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关键词： co-operate; motility; regulatory; single-domain response regulator; Xanthomonas

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.52； 五年影响因子：6.25 ）

ISSN： 1464-6722

年卷期： 2022 年 23 卷 5 期

页码：

收录情况： SCI

摘要： Signal transduction pathways mediated by sensor histidine kinases and cognate response regulators control a variety of physiological processes in response to environmental conditions in most bacteria. Comparatively little is known about the mechanism(s) by which single-domain response regulators (SD-RRs), which lack a dedicated output domain but harbour a phosphoryl receiver domain, exert their various regulatory effects in bacteria. Here we have examined the role of the SD-RR proteins encoded by the phytopathogen Xanthomonas campestris pv. campestris (Xcc). We describe the identification and characterization of a SD-RR protein named McvR (motility, chemotaxis, and virulence-related response regulator) that is required for virulence and motility regulation in Xcc. Deletion of the mcvR open reading frame caused reduced motility, chemotactic movement, and virulence in Xcc. Global transcriptome analyses revealed the McvR had a broad regulatory role and that most motility and pathogenicity genes were down-regulated in the mcvR mutant. Bacterial two-hybrid and protein pull-down assays revealed that McvR did not physically interact with components of the bacterial flagellum but interacts with other SD-RR proteins (like CheY) and the subset of DNA-binding proteins involved in gene regulation. Site-directed mutagenesis and phosphor-transfer experiments revealed that the aspartyl residue at position 55 of the receiver domain is important for phosphorylation and the regulatory activity of McvR protein. Taken together, the findings describe a previously unrecognized class of SD-RR protein that contributes to the regulation of motility and virulence in Xcc.

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