Biotransformation of soybean isoflavones by a marine Streptomyces sp 060524 and cytotoxicity of the products
文献类型: 外文期刊
第一作者: Hu, S. -C.
作者: Hu, S. -C.;Song, Y. -C.;Liu, J. -Y.;Tan, R. -X.;Hu, S. -C.;Hong, K.;Hu, S. -C.
作者机构:
关键词: biotransformation
期刊名称:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:3.312; 五年影响因子:3.58 )
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收录情况: SCI
摘要: A marine Streptomyces sp. 060524 capable of hydrolyzing the glycosidic bond of isoflavone glycosides, was isolated by detecting its beta-glucosidase activity. 5 isoflavone aglycones were isolated from culture filtrates in soybean meal glucose medium. They were identified as genistein (1), glycitein (2), daidzein (3), 3',4',5,7-tetrahydroxyisoflavone (4), and 3',4',7-trihydroxyisoflavone (5), based on UV, NMR and mass spectral analysis. The Streptomyces can selectively hydroxylate at the 3'-position in the daidzein and genistein to generate 3'-hydroxydaidzein and 3'-hydroxygenistein, respectively. The Strain biotransformed more than 90% of soybean isoflavone glycosides into their aglycones within 108 h. 3'-hydroxydaidzein and 3'-hydroxygenistein exhibited stronger cytotoxicity against K562 human chronic leukemia than daidzein and genistein.
分类号: Q93
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