Nanopriming with selenium doped carbon dots improved rapeseed germination and seedling salt tolerance

文献类型： 外文期刊

第一作者： Khan, Mohammad Nauman

作者： Khan, Mohammad Nauman;Fu, Chengcheng;Li, Yanhui;Yan, Jiasen;Yue, Lin;Li, Jiaqi;Wu, Honghong;Fu, Chengcheng;Yue, Lin;Wu, Honghong;Khan, Mohammad Nauman;Nie, Lixiao;Liu, Xiaohui;Khan, Zaid;Wu, Honghong;Wu, Honghong

作者机构：

关键词： PAA@Se-CDs; Nanopriming; Salt stress; Antioxidant system; ATP production; K*/Na* balance

期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）

ISSN： 2095-5421

年卷期： 2024 年 12 卷 5 期

页码：

收录情况： SCI

摘要： Soil salinity is a big environmental issue affecting crop production. Although seed nanopriming has been widely used to improve seed germination and seedling growth under salinity, our knowledge about the underlying mechanisms is still insufficient. Herein, we newly synthesized selenium-doped carbon dots nanoparticles coated with poly acrylic acid (poly acrylic acid coated selenium doped carbon dots, PAA@Se-CDs) and used it to prime seeds of rapeseeds. The TEM (transmission electron microscope) size and zeta potential of PAA@Se-CDs are 3.8 +/- 0.2 nm and- 30 mV, respectively. After 8 h priming, the PAA@Se-CDs nanoparticles were detected in the seed compartments (seed coat, cotyledon, and radicle), while no such signals were detected in the NNP (no nanoparticle control) group (SeO2 was used as the NNP). Nanopriming with PAA@Se-CDs nanoparticles increased rapeseeds germination (20%) and seedling fresh weight (161%) under saline conditions compared to NNP control. PAA@Se-CDs nanopriming significantly enhanced endo-b-mannanase activities (255% increase, 21.55 mu mol h- 1 g-1 vs. 6.06 mu mol h-1 g-1, at DAS 1 (DAS, days after sowing)), total soluble sugar (33.63 mg g-1 FW (fresh weight) vs. 20.23 mg g-1 FW) and protein contents (1.96 mu g g-1 FW vs. 1.0 mu g g-1 FW) to support the growth of germinating seedlings of rapeseeds under salt stress, in comparison with NNP control. The respiration rate and ATP content were increased by 76% and 607%, respectively. The oxidative damage of salinity due to the over- accumulation of reactive oxygen species (ROS) was alleviated by PAA@Se-CDs nanopriming by increasing the antioxidant enzyme activities (SOD (superoxide dismutase), POD (peroxidase), and CAT (catalase)). Another mechanism behind PAA@Se-CDs nanopriming improving rapeseeds salt tolerance at seedling stage was reducing sodium (Na*) accumulation and improving potassium (K*) retention, hence increasing the K*/Na* ratio under saline conditions. Overall, our results not only showed that seed nanopriming with PAA@Se-CDs could be a good approach to improve salt tolerance, but also add more knowledge to the mechanism behind nanopriming-improved plant salt tolerance at germination and early seedling growth stage. (c) 2024 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC- ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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