Transcriptomics reveals the mechanism of action of ultrasonic treatment to promote mung bean(Vigna radiata L.) sprouting

文献类型： 外文期刊

第一作者： Lu, Ming

作者： Lu, Ming;Xu, Yinghao;Wang, Man;Xu, Yinghao;Xu, Yinghao

作者机构：

关键词： Mung bean sprouts; Nutrient composition; Germination; Ultrasound; Transcriptomics

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.6； 五年影响因子：6.9 ）

ISSN： 0023-6438

年卷期： 2025 年 219 卷

页码：

收录情况： SCI

摘要： The objective of this study was to investigate the germination status and nutrient composition of four mung bean varieties (PB05, PB08, PB10 and 10L717) using the ultrasound-assisted mung bean germination method. The findings indicated that ultrasound can promote mung bean germination and improve its nutritional value. Furthermore, the optimal conditions were determined through one-way experimental optimisation and response surface analysis. Additionally, a transcriptomic analysis was conducted, which revealed that the pivotal genes involved in the pertinent metabolic pathways exhibited differential expression. This finding was subsequently validated through RT-qPCR. Furthermore, in order to gain deeper insight into the underlying mechanism of ultrasound-mediated mung bean sprout germination, we have identified and characterised the key transcription factor VrbZIP34. To elucidate the mechanism of action of ultrasound in affecting mung bean sprout germination, we have conducted a series of dual luciferase assay (DLR) experiments to investigate the function of this transcription factor. In conclusion, the results of this experiment demonstrate that ultrasound-assisted mung bean germination can significantly improve the germination of mung bean sprouts. The underlying mechanism of action was analysed from the perspective of both transcriptomics and molecular biology.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序