Overcoming obstacles to interspecific hybridization between Gossypium hirsutum and G. turneri

文献类型： 外文期刊

第一作者： Chen, Yu

作者： Chen, Yu;Chen, Yu;Feng, Shouli;Zhao, Ting;Zhou, Baoliang

作者机构：

关键词： Chromosome doubling; Embryo rescue; Gossypium hirsutum; Interspecific hybridization; Molecular marker; Wild cotton

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2018 年 214 卷 2 期

页码：

收录情况： SCI

摘要： Gossypium turneri, a wild cotton species (2n = 2X = 26, D10D10) originating from Mexico, possesses invaluable characteristics unavailable in the cultivated tetraploid cotton gene pool, such as caducous involucels at anthesis, resistance to insects and tolerance to abiotic stresses. However, transferring desired characteristics from wild species into cultivated cotton is often fraught with diverse obstacles. Here, Gossypium hirsutum (as the maternal parent) and G. turneri were crossed in the Hainan Province of China, and the obtained hybrid seeds (2n = 3X = 39, ADD(10)) were treated with 0.075% colchicine solution for 48 h to double the chromosome complement in order to overcome triploid F-1 sterility and to generate a fertile hexaploid. Chromosome doubling was successful in four individuals. However, the new synthetic hexaploids derived from these individuals were still highly sterile, and no seeds were generated by selfing or crossing. Therefore, an embryo rescue technique was employed in an attempt to produce progenies from the new synthetic hexaploids. Consequently, a total of six large embryos were obtained on MSB2K medium supplemented with 0.5 mg l(-1) KIN and 250 mg l(-1) CH using ovules from backcrossing that were 3 days post-anthesis. Four grafted surviving seedlings were confirmed to be the progenies (pentaploids) of the new synthetic hexaploids using cytological observations and molecular markers. Eight putative fertile individuals derived from backcrossing the above pentaploids were confirmed using SSR markers and generated an abundance of normal seeds. This research lays a foundation for transferring desirable characteristics from G. turneri into upland cotton.

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