Functional analysis of a rice 12-oxo-phytodienoic acid reductase gene (OsOPR1) involved in Cd stress tolerance

文献类型： 外文期刊

第一作者： Wu, Longying

作者： Wu, Longying;Wang, Ruolin;Li, Mingyu;Du, Zhiye;Jin, Yufan;Shi, Yang;Jiang, Wenjun;Jiao, Yuan;Huang, Jin;Chen, Ji;Hu, Binhua

作者机构：

关键词： Cadmium; Rice; Yeast; Cadmium tolerance; Jasmonic acid

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.8； 五年影响因子：2.7 ）

ISSN： 0301-4851

年卷期： 2024 年 51 卷 1 期

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收录情况： SCI

摘要： Background The accumulation of cadmium (Cd) in plants may compromise the growth and development of plants, thereby endangering human health through the food chain. Understanding how plants respond to Cd is important for breeding low-Cd rice cultivars. Methods In this study, the functions of 12-oxo-phytodienoic acid reductase 1 (OsOPR1) were predicted through bioinformatics analysis. The expression levels of OsOPR1 under Cd stress were analyzed by using qRT-PCR. Then, the role that OsOPR1 gene plays in Cd tolerance was studied in Cd-sensitive yeast strain (ycf1), and the Cd concentration of transgenic yeast was analyzed using inductively coupled plasma mass spectrometry (ICP-MS). Results Bioinformatics analysis revealed that OsOPR1 was a protein with an Old yellow enzyme-like FMN (OYE_like_FMN) domain, and the cis-acting elements which regulate hormone synthesis or responding abiotic stress were abundant in the promoter region, which suggested that OsOPR1 may exhibit multifaceted biological functions. The expression pattern analysis showed that the expression levels of OsOPR1 were induced by Cd stress both in roots and roots of rice plants. However, the induced expression of OsOPR1 by Cd was more significant in the roots compared to that in roots. In addition, the overexpression of OsOPR1 improved the Cd tolerance of yeast cells by affecting the expression of antioxidant enzyme related genes and reducing Cd content in yeast cells. Conclusion Overall, these results suggested that OsOPR1 is a Cd-responsive gene and may has a potential for breeding low-Cd or Cd-tolerant rice cultivars and for phytoremediation of Cd-contaminated in farmland.

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