Antioxidant Activity of Gracilaria lemaneiformis Polysaccharide Degradation Based on Nrf-2/Keap-1 Signaling Pathway in HepG2 Cells with Oxidative Stress Induced by H2O2
文献类型： 外文期刊
第一作者： Long, Xiaoshan
作者： Long, Xiaoshan;Liu, Shucheng;Long, Xiaoshan;Hu, Xiao;Pan, Chuang;Xiang, Huan;Chen, Shengjun;Qi, Bo;Yang, Xianqing;Hu, Xiao;Pan, Chuang;Xiang, Huan;Chen, Shengjun;Qi, Bo;Yang, Xianqing;Hu, Xiao;Liu, Shucheng;Yang, Xianqing
作者机构：
关键词： G; lemaneiformis; polysaccharide; degradation; free radicals; antioxidant; signaling pathway
期刊名称：MARINE DRUGS （ 影响因子：6.085； 五年影响因子：6.044 ）
ISSN：
年卷期： 2022 年 20 卷 9 期
页码：
收录情况： SCI
摘要： The objective of this research was to investigate the antioxidant activity of Gracilaria lemaneiformis polysaccharide degradation and its underlying mechanism involved in the Nrf-2/Keap-1 signaling pathway in HepG2 cells with oxidative stress induced by H2O2. The result of the scavenging ability of free radicals showed that GLP-HV (polysaccharide degraded by H2O2-vitamin C (Vc)) performed a better scavenging ability than GLP (G. lemaneiformis polysaccharide). Moreover, the scavenging ability of polysaccharide to these free radicals from strong to weak was as follows: superoxide radical, ferric ion, ABTS(+), and DPPH radical, and their IC50 values were 3.56 +/- 0.0028, 4.97 +/- 0.18, 9.62 +/- 0.35, and 23.85 +/- 1.78 mg/mL, respectively. Furthermore, GLP-HV obviously relieved oxidative stress in HepG2 cells, which strengthened the activity of T-AOC, CAT, GSH-PX, and SOD, and diminished the intensity of MDA, intracellular ROS, and calcium ion based on the Nrf-2/Keap-1 signaling pathway. The PCR result revealed that polysaccharide upregulated the expression of the genes Nrf-2, HO-1, NQO-1, and ZO-1 and downregulated Keap-1. The correlation between chemical properties and antioxidant mechanism of GLP-HV was evaluated via a heat map. The results illustrated that reducing sugar and active groups presented a positive correlation, and molecular weight and viscosity exhibited a negative relation with antioxidant activity.
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