Construction of Recombinant Marek's Disease Virus Co-Expressing VP1 and VP2 of Chicken Infectious Anemia Virus
文献类型： 外文期刊
第一作者： Li, Kai
作者： Li, Kai;Liu, Yongzhen;Liu, Changjun;Zhang, Yanping;Cui, Hongyu;Qi, Xiaole;Wang, Suyan;Chen, Yuntong;Duan, Yulu;Gao, Yulong;Wang, Xiaomei;Li, Kai;Li, Kai;Zhang, Jiayong;Xu, Jia;Wang, Xiaomei
作者机构：
关键词： Marek's disease virus; chicken infectious anemia virus; VP1; VP2; vaccine
期刊名称：VACCINES （ 影响因子：3.4； 五年影响因子：3.7 ）
ISSN：
年卷期： 2024 年 12 卷 9 期
页码：
收录情况： SCI
摘要： The chicken infectious anemia virus (CIAV) has been reported in major poultry-producing countries and poses a significant threat to the poultry industry worldwide. In this study, two Marek's disease virus (MDV) recombinants, rMDV-CIAV-1 and rMDV-CIAV-2, were generated by inserting the CIAV VP1 and VP2 genes into the MDV vaccine strain 814 at the US2 site using the fosmid-based rescue system. For rMDV-CIAV-1, an internal ribosome entry site was inserted between VP1 and VP2, so that both proteins were produced from a single open reading frame. In rMDV-CIAV-2, VP1 and VP2 were cloned into different open reading frames and inserted into the MDV genome. The recombinant viruses simultaneously expressed VP1 and VP2 in infected chicken embryo fibroblasts and exhibited growth kinetics similar to those of the parent MDV. The two recombinant viruses induced antibodies against CIAV in chickens. A single dose of the recombinant viruses provided strong protection against CIAV-induced anemia in chickens. These recombinant VP1- and VP2-expressing MDVs are potential vaccines against CIAV in chickens.
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