Improving cold-adaptability of mesophilic cellulase complex with a novel mushroom cellobiohydrolase for efficient low-temperature ensiling

文献类型： 外文期刊

第一作者： Gong, Ming

作者： Gong, Ming;Wang, Ying;Bao, Dapeng;Jiang, Shan;Chen, Hongyu;Shang, Junjun;Wang, Xiaojun;Zou, Gen;Yu, Hnin Hnin

作者机构：

关键词： Cellulase; Cellobiohydrolase; Cold-active; Ensilage; Bioparts

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2023 年 376 卷

页码：

收录情况： SCI

摘要： Low ambient temperature poses a challenge for rice straw-silage processing in cold climate regions, as cold limits enzyme and microbial activity in silages. Here, a novel cold-active cellobiohydrolase (VvCBHI-I) was isolated from Volvariella volvacea, which exhibited outstanding cellobiohydrolase activity at 10-30 degrees C. The crude cellulase complex in the VvCBHI-I-expressing transformant T1 retained 50% relative activity at 10 degrees C, while the wildtype Trichoderma reesei showed <5% of the activity. VvCBHI-I greatly improved the saccharification efficiency of the cellulase complex with pretreated rice straw as substrate at 10 C. In rice straw silage, pH (<4.5) and lactic acid content (>4.6%) remained stable after 15-day ensiling with the cellulase complex from T1 and Lactobacillus plantarum. Moreover, the proportions of cellulose and hemicellulose decreased to 29.84% & PLUSMN; 0.15% and 21.25% & PLUSMN; 0.26% of the dried material. This demonstrates the crucial potential of mushroom-derived cold active cellobiohydrolases in successful ensiling in cold regions.

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