Effects of energy-gathered and energy-divergent ultrasound on structure, DNA extraction and adulterated quantification of sweet potato vermicelli and its starch
文献类型： 外文期刊
第一作者： Muyembe, Diana Kavidia
作者： Muyembe, Diana Kavidia;Zhang, Miao;Sun, Hong-Nan;Mu, Tai-Hua
作者机构：
关键词： DNA extraction; sweet potato vermicelli; sweet potato starch; structure; ultrasound
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE （ 影响因子：3.5； 五年影响因子：4.2 ）
ISSN： 0022-5142
年卷期： 2025 年 105 卷 4 期
页码：
收录情况： SCI
摘要： BACKGROUNDThe identification of adulterated sweet potato vermicelli faces significant challenges, seriously hindering the development of the vermicelli industry. Herein, we investigate effects of energy-gathered ultrasound (EGU) and energy-divergent ultrasound (EDU) (30, 40 and 50 W L-1) on structure, DNA extraction and adulterated quantification of sweet potato vermicelli and its starch, thereby exploring their potential in adulteration of sweet potato vermicelli.RESULTSEGU-assisted modified cetyltrimethylammonium bromide (CTAB) protocol with beta-mercaptoethanol significantly improved DNA extraction from sweet potato vermicelli (223.7-249.2 ng mu L-1) and its starch (133.4-186.4 ng mu L-1), followed by EDU-assisted DNA extraction from sweet potato vermicelli (115.1-209.3 ng mu L-1) and its starch (33.4-61.0 ng mu L-1). Both EGU and EDU treatments resulted in the destruction of microstructure and crystalline structure, as well as changes in pasting and thermal properties of sweet potato vermicelli and its starch. Real-time polymerase chain reaction (PCR) amplification results revealed that EGU and EDU enhanced the efficiency of DNA amplification, and EDU showed smaller cycle threshold (Ct) values than EGU. In addition, EDU-assisted CTAB protocol combined with real-time PCR could detect levels of less than 1% of cassava and maize starches in sweet potato vermicelli.CONCLUSIONEDU-assisted CTAB protocol combined with real-time PCR shows promise as a sensitive and reliable analytical tool for vermicelli adulteration. (c) 2024 Society of Chemical Industry.
分类号：
- 相关文献
作者其他论文 更多>>
-
Effects of ultrasound with slightly acid electrolytic water on storage of sweet potato: Physiological, nutritional, sensory and microstructural characteristics
作者：Li, Lulu;Sun, Hong-Nan;Zhang, Miao;Mu, Tai-Hua
关键词：Sweet potato; Physiological property; Microstructure; Ultrasound; Slightly acid electrolytic water
-
Identification of targets and comparative study of administration methods for the lipid-lowering effects of fucoidan from Saccharina japonica
作者：Chen, Pengwei;Wang, Hao;Chen, Huiqin;Dai, Haofu;Hao, Li;Li, Quancai;Zhang, Miao;Jiang, Hongfei;Zhang, Renshuai;Jia, Ang
关键词：Fucoidan; Lipid-lowering activity; Dietary supplementation; Intestinal target; Gut microbiota
-
Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants
作者：Song, Xin;Zhang, Miao;Wang, Ting-Ting;Duan, Yao-Yuan;Ren, Jie;Gao, Hu;Fan, Yan-Jie;Xia, Qiang-Ming;Cao, Hui-Xiang;Xie, Kai-Dong;Wu, Xiao-Meng;Zhang, Fei;Tan, Feng-Quan;Guo, Wen-Wu;Song, Xin;Xia, Qiang-Ming;Zhang, Miao;Zhang, Si-Qi;Boualem, Adnane;Bendahmane, Abdelhafid;Tan, Feng-Quan;Zhang, Fei;Guo, Wen-Wu;Huang, Ying;Boualem, Adnane;Bendahmane, Abdelhafid
关键词：ethylene; perennial woody plant; polyploid; root architecture; salt tolerance
-
Are riparian buffer strips effective for nutrient retention under cold frozen conditions?
作者：Kumwimba, Mathieu Nsenga;Li, Xuyong;Huang, Jinlou;Kumwimba, Mathieu Nsenga;Li, Xuyong;Huang, Jinlou;Kumwimba, Mathieu Nsenga;Dzakpasu, Mawuli;Ifon, Binessi Edouard;Muyembe, Diana Kavidia;Akter, Salma;Zhang, Yufan;Guadie, Awoke;Manirakiza, Benjamin
关键词：Nitrogen and phosphorus; Vegetated filter strips; Warm and cold climates; Removal efficiency; Surface runoff; Watershed management; Freeze-thaw cycles
-
The potential of energy-divergent ultrasound, slightly acidic electrolyzed water and peracetic acid in sweet potato preservation to control Rhizopus stolonifer
作者：Liu, Guang-Chen;Sun, Hong-Nan;Zhang, Miao;Mu, Tai-Hua
关键词：Energy-divergent ultrasound; Peracetic acid; Slightly acidic electrolyzed water; Rhizopus stolonifer; Sweet potato; Storage quality
-
Development of a monoclonal antibody-based colloidal gold immunochromatographic strip for rapid detection of feline coronavirus
作者：Zhang, Miao;Zhu, Yingqi;Li, Na;Aishanjiang, Kelimujiang;Zhu, Shiqiang;Tang, Aoxing;Li, Guoxin;Liu, Guangqing
关键词：N protein; Monoclonal antibody; Colloidal gold-based immunochromatographic strip
-
Micro/nanobubble aeration promotes rapid and stable partial nitrification by strengthening the synergistic activity of functional microbial communities in the treatment of low-strength rural wastewater
作者：Kumwimba, Mathieu Nsenga;Li, Xuyong;Huang, Jinlou;Kumwimba, Mathieu Nsenga;Li, Xuyong;Huang, Jinlou;Kumwimba, Mathieu Nsenga;Li, Jing;Dzakpasu, Mawuli;Ifon, Binessi Edouard;Muyembe, Diana Kavidia
关键词：Partial nitrification (PN) system; Micro and nano bubble technology; Aeration performance; Real rural sewage treatment; Metagenomic analysis
