Effects of Ammonia Stress on the Antioxidant, Ferroptosis, and Immune Response in the Liver of Golden Pompano Trachinotus ovatus

文献类型： 外文期刊

第一作者： Duan, Yafei

作者： Duan, Yafei;Xiao, Meng;Zhu, Ruijie;Nan, Yuxiu;Yang, Yukai;Huang, Xiaohua;Zhang, Dianchang;Duan, Yafei;Zhang, Dianchang

作者机构：

关键词： fish; ammonia; liver; antioxidant; ferroptosis; immunity

期刊名称：ANTIOXIDANTS （ 影响因子：6.6； 五年影响因子：7.3 ）

ISSN：

年卷期： 2025 年 14 卷 4 期

页码：

收录情况： SCI

摘要： Ammonia is the main harmful environmental substance affecting fish culture. The liver is the immune and metabolic organ of fish, and its physiological homeostasis will affect the health of the organism. In this study, healthy golden pompano Trachinotus ovatus juveniles were exposed to 5 mg/L (A5) and 10 mg/L (A10) ammonia-N stress for 7 days and then the variation characteristics of the physiological homeostasis of the liver were analyzed at multiple biological levels. After ammonia stress, the liver showed obvious morphological changes and stress responses. Specifically, the oxidative stress indexes, such as the activities of the anti-superoxide anion generation capacity (ASC) and superoxide dismutase (SOD), were elevated in the A5 and A10 groups, while the glutathione peroxidase (GPx) activity and glutathione (GSH) content were disturbed; the relative expression levels of the Nrf2 and NQO1 genes were increased in the A10 group, while the expressions of the Keap1 and HO1 were decreased in the A5 and A10 groups. Ferroptosis related genes, such as the relative expressions of NOX1, NCOA4, and FPN1 were increased in the A5 and A10 groups, PTGS2 and FTH1 were decreased in the A5 group but elevated in the A10 group, and p53, GPx4, SLC7A11, and NFS1 were only increased in the A10 group. Inflammation related genes, such as TNF alpha, IL1 beta, and IL8 relative expression levels, were increased in the A10 group, IL10 was increased in the A5 and A10 groups, while TGF beta was decreased in the A5 group but increased in the A10 group. Immune related genes, such as the expression levels of IgM and IgT, were increased in the A5 group but decreased in the A10 group. The integrated biomarker responses revealed that the hepatotoxicity of ammonia was concentration-dependent, and there was a high correlation between oxidative stress, ferroptosis, inflammation, and immune function changes. These results reveal the hepatotoxicity of ammonia stress on T. ovatus.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序