SPECIFIC AND SENSITIVE DETECTION OF THE FUNGAL PATHOGEN USTILAGINOIDEA VIRENS BY NESTED PCR

文献类型： 外文期刊

第一作者： Zhou Yongli

作者： Zhou Yongli

作者机构：

关键词： Rice false smut;ITS;PCR primers

期刊名称：菌物学报

ISSN： 1672-6472

年卷期： 2004 年 23 卷 1 期

页码：

收录情况： CSCD

摘要： A 624-625 bp fragment of the inner space region of ITS and 5.8S rDNA of strains of Ustilaginoidea virens were sequenced, which collected from diverse rice varieties and geographical origins in different years in order to design PCR primers for its detection. Alignment of the sequence data of all collections revealed the ITS region of this pathogen was almost completely conservative. Based on the comparison with the ITS sequences of other Clavicipitaceous fungi, specific primers for U. virens were developed. The objective fungus was successfully detected with its specific primer set with high specificity and sensitivity, and the PCR results correlated well with assessments based on conventional technique of histological inspection. This result could provide a useful technique to study disease cycle and early prediction of field incidence of false smut.

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