Vitamin determination in pet food: A fast and sensitive online solid-phase extraction and heart-cutting 2D liquid chromatography method
文献类型： 外文期刊
第一作者： Li, Yang
作者： Li, Yang;Feng, Yuchao;Chen, Xue;Cui, Jie;Fan, Xia;Wang, Jitong;Yao, Ting;Gong, Ping;Gao, Xing;Guo, Wei
作者机构：
关键词： Vitamin A; Vitamin D; Vitamin E isomers; Pet food; Online solid-phase extraction; Two-dimensional liquid chromatography
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）
ISSN： 0026-265X
年卷期： 2025 年 213 卷
页码：
收录情况： SCI
摘要： An automatic method that combines online solid-phase extraction (online SPE) and heart-cutting two-dimensional liquid chromatography (2D-LC) was developed to determine seven fat-soluble vitamins simultaneously and was validated in pet food. The saponified sample solution was injected directly into our combined online SPE and heart-cut 2D-LC system for enrichment, purification, separation, and quantification. Vitamin A and E isomers were analyzed using pentafluorophenyl (PFP) as the first column. Vitamins D-2 and D-3 were analyzed using C-18 as the second column. The results showed good linearity (R-2 > 0.9964) for the analytes and good reproducibility (RSD < 3 %, n = 3). The limits of quantification for vitamins A, D-2, and D-3, and alpha -, beta-, gamma-, and delta-E (calculated using S/N = 10:1) were 0.24, 0.09, 0.09, 2.4, 2.4, 2.4 and 2.4 mu g/g, respectively. The spiked recoveries were from 80.6 to 104.0 %. The proposed method considerably simplifies the analytical workflow of standard methods for the analysis of fat-soluble vitamins and improves efficiency.
分类号：
- 相关文献
作者其他论文 更多>>
-
A rapid and highly sensitive CRISPR assay utilizing Cas12a orthologs for the detection of Novel Duck Reovirus
作者：Wang, Yuan;Fu, Lanting;Li, Sheng;Tao, Dagang;Ruan, Jinxue;Xie, Shengsong;Wang, Yuan;Fu, Lanting;Li, Sheng;Tao, Dagang;Ruan, Jinxue;Xie, Shengsong;Wang, Cui;He, Daqian;Gong, Ping;Yang, Yu
关键词：NDRV; Cas12a orthologs; Gs12-16; Gs12-18; CRISPR-based diagnostics
-
Comparative transcriptome analysis of gill tissue response to hypoxia stress in the Chinese mitten crab ( Eriocheir sinensis)
作者：Chen, Xue;Bai, Yulin;Wang, Anqi;Xu, Dongpo;Ma, Junlei;Xu, Dongpo
关键词：Transcriptome; Eriocheir sinensis; Hypoxia; Oxidative stress; Apoptosis
-
Diversification and evolution of serine/arginine-rich splicing factors in plants
作者：Guo, Mingxi;Liang, Chao;Cui, Mingyang;Luo, Linlin;Gao, Zhongtian;Chen, Haolang;Xie, Qi;Cui, Jie;Liu, Lin;Mo, Beixin;Gao, Lei;Luo, Linlin;Xie, Qi
关键词：SRSFs; alternative splicing; phylogenetic analysis; molecular evolution; origin; diversification
-
A LTR retrotransposon insertion leads to leafy phenotype in maize by elevating ZmOM66 expression
作者：Du, Xuemei;Chen, Yan;Gao, Xinpeng;Wang, Xiaoli;Cui, Yu;Liu, Yunjun;Fu, Junjie;Wang, Guoying;Du, Xuemei;Xu, Zhuoyi;Lu, Jiawen;Zhang, Jie;He, Cheng;Huang, Liying;Guo, Wei;Cui, Yangbo;Ai, Junmin;Li, Li;Gu, Riliang;Wang, Jianhua
关键词：
-
A comprehensive review of starch-based technology for encapsulation of flavor: From methods, materials, and release mechanism to applications
作者：Shan, Yimeng;Li, Jiaxin;Nie, Mengzi;Li, Dezhi;Zhang, Yue;Li, Yang;Wang, Lili;Liu, Liya;Wang, Fengzhong;Tong, Li-Tao;Tong, Li-Tao
关键词：Encapsulation techniques; Flavor; Starch-based materials; Inclusion complex; Release mechanisms; Industry applications
-
Numerical Simulation Analysis and Research on Drag Reduction and Stability Enhancement Effect of Head Swing Based on Biomimetic Flexible Variants
作者：Qi, Debo;Li, Yang;Yang, Baisheng;Wu, Zhengyang;Li, Bo;Niu, Shichao
关键词：head swing; drag reduction; biomimetic flexible variants; radial drag; numerical simulation
-
BRASSINAZOLE RESISTANT 1 delays photoperiodic flowering by repressing CONSTANS transcription
作者：Xu, Xingwen;Chen, Yangbo;Tian, Hao;Yang, Zijian;Liu, Shuo;Li, Xiaopeng;Song, Chunhui;Ye, Zhangli;Guo, Wei;Kong, Dongdong;Hou, Congcong;Li, Legong;Liu, Liangyu;Xu, Xingwen;Chen, Yangbo;Tian, Hao;Yang, Zijian;Liu, Shuo;Li, Xiaopeng;Song, Chunhui;Ye, Zhangli;Guo, Wei;Kong, Dongdong;Hou, Congcong;Li, Legong;Liu, Liangyu;Jiang, Wenbo
关键词：
