A 90-Day Safety Study of Meat from MSTN and FGF5 Double-Knockout Sheep in Wistar Rats

文献类型： 外文期刊

第一作者： Zhao, Yue

作者： Zhao, Yue;Chen, Mingming;Li, Yao;Xu, Xueling;Wu, Sujun;Liu, Zhimei;Qi, Shiyu;Yi, Guang;Yu, Kun;Deng, Shoulong;Li, Yan;Lian, Zhengxing;Zhang, Xiaosheng;Zhang, Jinlong;Guo, Xiaofei;Deng, Shoulong;Deng, Shoulong

作者机构：

关键词： meat; MSTN; FGF5; sheep; 90-day feeding; rat

期刊名称：LIFE-BASEL （ 影响因子：3.251； ）

ISSN：

年卷期： 2022 年 12 卷 2 期

页码：

收录情况： SCI

摘要： MSTN and FGF5 gene knockout sheep generated by the CRISPR/Cas9 system exhibit the 'double-muscle' phenotype, and increased density and length of hairs, providing valuable new breeding material. In a previous study, we obtained MSTN and FGF5 double-knockout sheep of significant breeding value. In this study, we carried out a 90-day feeding study in Wistar rats to assess the safety of genome-edited mutton. Seven rat groups with 10 females and 10 males per group were fed different concentrations (3.75%, 7.5%, and 15%) of double-knockout mutton or wild-type mutton in a conventional commercial diet for 90 days. At the end of the feeding, routine urine and blood tests and measurements of blood biochemical indicators were performed. Furthermore, the major organs of each group of rats were weighed and examined histopathologically. Although there were significant differences among the groups in some parameters, all values were within the normal ranges. Therefore, the 90-day rat feeding study showed that the meat from MSTN and FGF5 double-knockout sheep did not have any long-term adverse effects on rat health. This study also provides valuable reference information for assessing the safety of meat from animals with knockout of multiple genes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序