Development a high-sensitivity sandwich ELISA for determining antigen content of porcine circovirus type 2 vaccines

文献类型： 外文期刊

第一作者： Xu, Lele

作者： Xu, Lele;Chen, Zhihao;Gong, Haoyang;Pei, Xiuxiu;Zhu, Yiyao;Lu, Yuchen;Wang, Yumiao;Nan, Shifa;Yin, Yupeng;Zhao, Qin;Fan, Yunpeng;Sun, Yani;Xu, Lele;Chen, Zhihao;Gong, Haoyang;Pei, Xiuxiu;Zhu, Yiyao;Lu, Yuchen;Wang, Yumiao;Nan, Shifa;Xiao, Shuqi

作者机构：

关键词： Porcine circovirus type 2; Vaccine; DAS-ELISA; Quality control

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.2； 五年影响因子：2.0 ）

ISSN： 0166-0934

年卷期： 2024 年 328 卷

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收录情况： SCI

摘要： Porcine circovirus type 2 (PCV2) is intensely prevalent in global pig farms. The PCV2 vaccine is an important means of preventing and controlling PCV2. The quality control of PCV2 vaccines is predominantly based on detection techniques such as animal testing and neutralizing antibody titration. Measuring the content of effective proteins in vaccines to measure vaccine efficacy is an excellent alternative to traditional methods, which can greatly accelerate the development speed and testing time of vaccines. In this study, we screened a monoclonal antibody (mAb) that can effectively recognize not only the exogenous expression of PCV2 Cap protein but also PCV2 virus. The double antibody sandwich ELISA (DAS-ELISA) was developed using this mAb that specifically recognize PCV2 Cap. The minimum protein content detected by this method is 3.5 ng/mL. This method can be used for the quality control of PCV2 inactivated vaccine and subunit vaccine, and the detection results are consistent with the results of mice animal experiments. This method has the advantages of simple operation, good sensitivity, high specificity and wide application. It can detect the effective antigen Cap protein content of various types of PCV2 vaccines, which not only shorten the vaccine inspection time but also save costs.

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