The potential application of isoxanthohumol in inhibiting Clostridium perfringens infection by targeting the type IV pili

文献类型： 外文期刊

第一作者： Song, Zeyu

作者： Song, Zeyu;Deng, Yanhong;Zhang, Jichuan;Deng, Xuming;Zhang, Qiaoling;Lv, Qianghua;Wen, Zhongmei;Liu, Shui;Lv, Qianghua;Lv, Qianghua

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关键词： C. perfringens; Zoonotic pathogen; Isoxanthohumol; TFP; Anti-infection

期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：4.9； 五年影响因子：6.1 ）

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年卷期： 2025 年 24 卷 1 期

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收录情况： SCI

摘要： BackgroundClostridium perfringens (C. perfringens) is an important zoonotic pathogen. The diseases such as necrotic enteritis (NE), enterotoxemia, gas gangrene and food poisoning caused by its infection seriously threaten the lives of both humans and animals. However, under the severe situation of antibiotic resistance, the development of new antibacterial strategies or drugs deserves great attention.ResultsIn this study, we selected the virulence factor Type IV pili (TFP) of C. perfringens as the target for drug screening. The gliding motility, biofilm formation, cell adhesion and antibacterial activity of the natural compound isoxanthohumol (IXN) against C. perfringens were determined. Transmission electron microscopy (TEM), TFP gene transcription analysis and Western blot were used to detect the expression of PilA pilin. The therapeutic effect of IXN on C. perfringens infection was demonstrated through a mouse gas gangrene model. It was confirmed that IXN inhibits the function of TFP by down-regulating TFP-encoding genes and two-component regulatory genes.ConclusionsIn conclusion, our study shows that IXN has the potential to inhibit the function of TFP in C. perfringens and for anti-infection applications.

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