RNAi-mediated silencing of SlitPer disrupts sex pheromone communication behavior in Spodoptera litura

文献类型： 外文期刊

第一作者： Zhang, Ya-Nan

作者： Zhang, Ya-Nan;Sun, Liang;Yang, Hui-Hui;Li, Jian-Qiao;Ma, Sai;Yao, Wei-Chen;Chen, Yu-Wen;Zhu, Xiu-Yun;Zhang, Ya-Nan;El Wakil, Abeer;Dewer, Youssef;Sun, Liang

作者机构： Huaibei Normal Univ, Coll Life Sci, Huaibei 235000, Peoples R China;Chinese Acad Agr Sci, Tea Res Inst, Key Lab Tea Qual & Safety Control, Minist Agr & Rural Affairs, Hangzhou 310008, Peoples R China;Huaibei Normal Univ, Coll Life Sci, Huaibei, Peoples R China;Alexandria Univ, Fac Educ, Biol & Geol Sci Dept, Alexandria, Egypt;Agr Res Ctr, Phytotox Res Dept, Cent Agr Pesticide Lab, Giza, Egypt;Chinese Acad Agr Sci, Tea Res Inst, Key Lab Tea Qual & Safety Control, Minist Agr & Rural Affairs, Hangzhou, Peoples R China

关键词： Spodoptera litura; circadian rhythm; period; sex pheromone communication; RNAi

期刊名称：PEST MANAGEMENT SCIENCE （ 2022影响因子：4.1； 五年影响因子：4.4 ）

ISSN： 1526-498X

年卷期： 2023 年

页码：

收录情况： SCI

摘要： BackgroundThe 24-h circadian rhythm is considered crucial for insect sexual communication. However, its molecular mechanisms and signaling pathways, particularly the roles of the clock gene period (Per), remain largely unclear. The sex pheromone communication behavior of Spodoptera litura displays typical circadian rhythm characteristics. Thus, it represents an excellent model for functional analyses of the clock gene Per. ResultsIn this study, we investigated the potential roles of SlitPer in regulating sex pheromone communication in S. litura using RNA interference, quantitative real-time polymerase chain reactions (qPCR), gas chromatography, and behavioral assays. The qPCR results showed that the expression levels of SlitPer and two desaturase genes (SlitDes5 and SlitDes11) in the siPer group differed significantly at most time points from those in the siNC group. Dynamic variation in the three major sex pheromone titers and calling behavior of S. litura females in the siPer group was disordered. In addition, the mating rates of siPer S. litura females decreased significantly by 33.33%. Oviposition by mated siPer females was substantially reduced by 84.84%. ConclusionThese findings provide a fundamental basis for elucidating the molecular mechanism by which Per regulates sex pheromone communication behavior in lepidopteran species. (c) 2023 Society of Chemical Industry.

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