An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development

文献类型： 外文期刊

第一作者： Guo, Songxin

作者： Guo, Songxin;Zhang, Hui;Men, Dong;Cui, Zongqiang;Ma, Chun;Gong, Jun;Xu, Jintian;Zhang, Xiaowei;Rong, Heng;Wang, Yan-Yi;Deng, Jiaoyu;Li, Feng;Guo, Songxin;Cui, Zongqiang;Ma, Chun;Gong, Jun;Xu, Jintian;Wang, Dianbing;Gong, Rui;Rong, Heng;Wang, Yan-Yi;Deng, Jiaoyu;Li, Feng;Ru, Yi;Liu, Huanan;Shen, Chaochao;Tian, Hong;Zheng, Haixue;Xue, Junbiao;Tao, Shengce;Men, Dong;Wang, Dianbing;Gong, Rui;Zhang, Xian-En;Liu, Chenli;Dai, Zhuojun;Zhang, Xian-En

作者机构：

关键词： African swine fever virus; proteome microarray; humoral immunity; live-attenuated vaccines; B-cell antigens

期刊名称：JOURNAL OF VIROLOGY （ 影响因子：3.8； 五年影响因子：3.9 ）

ISSN： 0022-538X

年卷期： 2025 年

页码：

收录情况： SCI

摘要： Developing an efficient and safe vaccine for African swine fever (ASF), a devastating disease of pigs, remains a significant challenge mainly due to limited knowledge of the immune correlates of protection. Identifying protective determinants is difficult because ASF virus (ASFV) is a large and complex DNA virus encoding over 160 proteins. Here, we constructed an ASFV proteome microarray containing 160 full-length proteins for profiling ASFV-specific antibodies. An antibody reactome containing 46 ASFV proteins (including 12 newly recognized B-cell antigens) was established by analyzing several cohorts of serum samples from pigs protected with different live-attenuated vaccines (LAVs). A proteome-wide study of antibody dynamics over a 26-day period provided a multi-dimensional landscape of the host humoral response against ASFV after acute infection, LAV immunization, and post-vaccination challenge. This study provides a comprehensive understanding of ASFV-induced humoral immune responses, highlights B-cell antigen candidates for vaccine design, supports the investigation of LAV protection mechanisms, and would accelerate vaccine development.IMPORTANCEAfrican swine fever (ASF) poses a severe threat to global swine industries, with vaccine development hindered by limited understanding of immune protection. A comprehensive understanding of antibody responses against ASF virus (ASFV) and the discovery of protective antigens are fundamental to vaccine development. This study constructed an ASFV proteome microarray to profile antibody responses against 160 viral proteins and established the antibody spectra against ASFV with dynamic features. The proteome microarray offers a high-throughput platform for understanding ASFV immunology and pathogenicity and will contribute to ASF vaccine development and diagnosis.

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