Spatial distribution of maternal factors in pig mature oocytes

文献类型： 外文期刊

第一作者： Zhu, Fuquan

作者： Zhu, Fuquan;Yang, Meng;Wang, Dayu;Jiang, Yuan;Jia, Chao;Yu, Aochen;Liu, Honglin;Li, Juan;Fu, Yanfeng;Liu, Huijun;Wang, Meixia;Wang, Tingzhang

作者机构：

关键词： Porcine; transcriptomics; proteomics; maternal factors; oriented bisection

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：1.8； 五年影响因子：2.0 ）

ISSN： 1049-5398

年卷期： 2024 年 35 卷 1 期

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收录情况： SCI

摘要： It is known that asymmetrical maternal transcripts play an important role in the cell fate of the early embryo, but few studies are available in mammal oocytes especially in pig. To investigate the spatial factors in pig oocytes, the oriented bisection was established for collecting karyoplasts (NSOs) and cytoplasts (SSOs) with more than 95% efficiency. Subsequently, RNA-Seq and LC-MS/MS analysis were performed on NSOs and SSOs. Although no differentially expressed genes (DEGs) could be detected between NSOs and SSOs, 89 of the differentially expressed proteins (DEPs) were detected, that 58 proteins higher expressed but 31 proteins lower expressed in NSOs compared with SSOs. These DEPs mainly participated in the 'cell cycle' and 'ribosome' pathway, while the up-regulated DEPs were mainly GO in 'spindle' and 'positive regulation of translation', and the down-regulated DEPs were in 'cytosolic small ribosomal subunit' and 'mRNA binding'. The up-regulated DEP SIRT5 which are related to the regulation of gene expression, epigenetic were further detected and revealed. A spatial asymmetry of maternal factors at the protein level was firstly detected in pig mature oocytes.

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