The Naturally Occurring Amino Acid Substitution in the VPg α1-α2 Loop Breaks eIF4E-Mediated Resistance to PRSV by Enabling VPg to Re-Hijack Another eIF4E Isoform eIF(iso)4E in Watermelon
文献类型： 外文期刊
第一作者： Zhou, Ling-Xi
作者： Zhou, Ling-Xi;Yan, Zhi-Yong;Jiang, Jun;Tian, Yan-Ping;Geng, Chao;Li, Xiang-Dong;Yin, Xiao;Li, Xiang-Dong;Gao, Rui
作者机构：
关键词： eIF(iso)4E; eIF4E; PRSV-W; resistance breaking; VPg
期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.6 ）
ISSN： 1464-6722
年卷期： 2024 年 25 卷 11 期
页码：
收录情况： SCI
摘要： Plant resistance, which acts as a selective pressure that affects viral population fitness, leads to the emergence of resistance-breaking virus strains. Most recessive resistance to potyviruses is related to the mutation of eukaryotic translation initiation factor 4E (eIF4E) or its isoforms that break their interactions with the viral genome-linked protein (VPg). In this study, we found that the VPg alpha 1-alpha 2 loop, which is essential for binding eIF4E, is the most variable domain of papaya ringspot virus (PRSV) VPg. PRSV VPg with the naturally occurring amino acid substitution of K105Q or E108G in the alpha 1-alpha 2 loop fails to interact with watermelon (Citrullus lanatus) eIF4E but interacts with watermelon eIF(iso)4E instead. Moreover, PRSV carrying these mutations can break the eIF4E-mediated resistance to PRSV in watermelon accession PI 244019. We further revealed that watermelon eIF(iso)4E with the amino acid substitutions of DNQS to GAAA in the cap-binding pocket could not interact with PRSV VPg with natural amino acid substitution of K105Q or E108G. Therefore, our finding provides a precise target for engineering watermelon germplasm resistant to resistance-breaking PRSV isolates.
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