Fabrication of resveratrol-loaded soy peptide nanogels with transglutaminase and in vitro gastrointestinal delivery and cholesterol-lowering effect

文献类型： 外文期刊

第一作者： Fang, Mengxue

作者： Fang, Mengxue;Ma, Fei;Yu, Li;Wang, Du;Zhang, Liangxiao;Li, Peiwu;Fang, Mengxue;Ma, Fei;Yu, Li;Wang, Du;Zhang, Liangxiao;Li, Peiwu;Fang, Mengxue;Ma, Fei;Yu, Li;Wang, Du;Zhang, Liangxiao;Li, Peiwu;Fang, Mengxue;Ma, Fei;Yu, Li;Wang, Du;Zhang, Liangxiao;Li, Peiwu;Zhang, Liangxiao;Li, Peiwu;Zhang, Liangxiao;Zhang, Liangxiao;Li, Peiwu;Wen, Chaoting

作者机构：

关键词： Peptide; Nanogel; Resveratrol; Transglutaminase; Digestion; Cholesterol-lowering

期刊名称：FOOD HYDROCOLLOIDS （ 影响因子：11.0； 五年影响因子：11.3 ）

ISSN： 0268-005X

年卷期： 2024 年 157 卷

页码：

收录情况： SCI

摘要： To improve the intestinal delivery of resveratrol, soy protein peptides (SPP) and transglutaminase (TGase) were used to prepare nanogels (NGs). The results demonstrated that, compared with native soy protein isolate (SPI) NG, SPP-NG showed a lower encapsulation efficiency (EE, 70.26%), a higher release efficiency (RE, 95.83%), and a smaller particle size (194.43 nm). However, the addition of TGase increased the EE and particle size to 74.37% and 217.73 nm, respectively. The RE of SPP-TGNG was also maintained at as high as 93.28%. Interestingly, SPPTGNG exhibited optimal bile acid-binding capacity and cholesterol-lowering digests. The differences in delivery effects and morphology were attributed to structural changes in NGs. The unfolding and exposure of hydrophobic residues and weakened hydrogen bonds by hydrolysis resulted in a disordered and loose network structure, but it was beneficial to the production of cholesterol-lowering peptides and amino acids after digestion. The presence of TGase strengthened the crosslinking network by increasing beta-sheet contents, hydrogen bonds, hydrophobic interactions, and isopeptide bonds. The alteration in multilevel structure would further affect the digestive products. The exposure of more hydrophobic residues and isopeptide crosslinking may be the intrinsic reason for the production of more hypocholesterolemic peptides and amino acids. This study indicated that the combination of hydrolysis and TGase treatment enhanced the delivery efficiency and bioactive properties of peptide-based NGs to resveratrol and revealed the internal mechanism between the structural changes and delivery efficiency. This work offers a theoretical foundation and technical suggestions for the production of peptide-based cargo systems and hydrophobic active substances.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序