ASFV transcription reporter screening system identifies ailanthone as a broad antiviral compound

文献类型： 外文期刊

第一作者： Zhang, Yuhang

作者： Zhang, Yuhang;Zheng, Xiaofeng;Zhang, Zhenjiang;Zhang, Jiwen;Zhao, Dongming;Zhang, Fan;Jiao, Jun;Hou, Min;Qian, Nianchao;Tan, Xu;Tan, Xu

作者机构：

关键词： African swine fever virus (ASFV); Replication inhibition; Broad-spectrum antiviral drugs; Ailanthone (AIL)

期刊名称：VIROLOGICA SINICA （ 影响因子：5.5； 五年影响因子：4.9 ）

ISSN： 1674-0769

年卷期： 2023 年 38 卷 3 期

页码：

收录情况： SCI

摘要： African swine fever (ASF) is an acute, highly contagious and deadly viral disease in swine that jeopardizes the worldwide pig industry. Unfortunately, there are no authoritative vaccine and antiviral drug available for ASF control. African swine fever virus (ASFV) is the etiological agent of ASF. Among the ASFV proteins, p72 is the most abundant component in the virions and thus a potential target for anti-ASFV drug design. Here, we con-structed a luciferase reporter system driven by the promoter of p72, which is transcribed by the co-transfected ASFV RNA polymerase complex. Using this system, we screened over 3200 natural product compounds and obtained three potent candidates against ASFV. We further evaluated the anti-ASFV effects and proved that among the three candidates, ailanthone (AIL) inhibits the replication of ASFV at the nanomolar concentration (IC50 1/4 15 nmol/L). Our in vitro experiments indicated that the antiviral effect of AIL is associated with its inhibition of the HSP90-p23 cochaperone. Finally, we showed the antiviral activity of AIL on Zika virus and hepatitis B virus (HBV), which supports that AIL is a potential broad-spectrum antiviral agent.

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