Hypothetical bromodomain-containing protein 5 is required for the growth of Toxoplasma gondii

文献类型： 外文期刊

第一作者： Zhang, Ying

作者： Zhang, Ying;Qiu, Hongyu;Deng, Ruipeng;Wang, Chunren;Zhang, Ying;Cheng, Long;Sun, Tiancong;Gong, Haiyan;Mi, Rongsheng;Huang, Yan;Chen, Zhaoguo;Zhang, Ying

作者机构：

关键词： Toxoplasma gondii; Bromodomain-containing protein 5; Localization; Proliferation; Conditional ablation

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.821； 五年影响因子：2.862 ）

ISSN： 0304-4017

年卷期： 2022 年 309 卷

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收录情况： SCI

摘要： Bromodomain (BRD) is a highly conserved structural module domain, found in various proteins, including chromatin-related proteins, nucleus acetyltransferases, and transcription-associated proteins. Toxoplasma gondii, a zoonotic protozoan, encodes at least 12 predicted BRD-containing proteins (BDPs). Here, we investigated the subcellular location and regulatory role of a hypothetical protein BDP that we named TgBDP5. The BRD of TgBDP5 did not contain the conserved Asn and Tyr residues required for acetyl-lysine recognition. TgBDP5 localized in the nucleus of the parasite and remained unchanged during parasite replication. Conditional ablation of TgBDP5 through an auxin-inducible degron-based knockdown strategy caused a growth defect in parasite replication. Depletion of TgBDP5 led to changes in the expression level of 179 genes, suggesting it as an important target for drugs acting against T. gondii.

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